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HPLC Application Notes

High-performance liquid chromatography (HPLC) applies to various industries, including pharmaceutical, environmental, industrial, and forensic sciences. Hamilton's HPLC library of application notes contains the information you need. View our industry-specific app notes and application notes for the Microlab 600 below.

HPLC Pharmaceutical Applications

In recent years, the number of HPLC pharmaceutical applications has continued to grow. From isolating potent chemotherapy agents to understanding weight non-reducing sugars, the pharmaceutical industry is pushing boundaries. Learn more through Hamilton's pharmaceutical HPLC applications.

Colorful chain of amino acids peptides

Separation of Biologically Active Increasing Length Peptides

This study highlights how the PRP-3 HPLC stationary phase can be a useful tool for separating biologically active peptides. These findings are on the cutting edge of developing new therapeutic healthcare products.

capsules in blue glass container

Separation of Basic Drug Compounds (PRP-C18)

Using the PRP-C18 HPLC column, this study dives deeper into how mobile phase pH was used to separate basic drug compounds.

HPLC Environmental Applications

Exploring the environmental applications of HPLC covers both naturally occurring chemicals and components, as well as our effect on nature. Topics surrounding the effects of psilocybin, BPA, and separation of oils all have an environmental impact.

Arsenic Speciation by Anion Exchange HPLC (PRP-X100)

Organic arsenic compounds are added to many animal feeds and can pose a significant ecological and environmental threat. Hamilton's PRP-X100 circumvents diverse sample matrices and provides a resolution for these compounds.

Hexavalent Chromium by Anion Exchange HPLC (PRP-X100)

Toxic levels of hexavalent chromium, a poison to humans and aquatic life, have been detected in several groundwater sources. Analysis of chromium species was challenging until a new method using Hamilton's PRP-X100 was developed.

Food & Beverage HPLC Applications

Everything we eat or consume has an impact on our health and continues to be a significant area of study. As a result, new methods of isolating and studying chemicals and compounds are needed, specifically for HPLC applications in food and beverages.

The Heat is On: The Isolation of Capsaicin for Therapeutic Treatments

Capsaicin, the defense mechanism of chili peppers, is now being investigated for therapeutic treatments. Utilizing Hamilton's PRP-C18 HPLC column, major capsaicinoids can now be isolated and studied.

Poisoned by Plastic: The Analysis of BPA and its Derivatives in Water Bottles

While plastic has minimal environmental and health costs, long-term unforeseen effects on the human body are beginning to be discovered. Studies are using HPLC detection to analyze the side effects of BPA toxicity.

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Applications of HPLC in Forensics Sciences

Hamilton continues to make strides in HPLC applications for forensic sciences. Separating and analyzing chemicals in drugs, explosives, and other forensics is relevant to criminology and human health.

Benzodiazepine Metabolites by Reversed-Phase HPLC (PRP-1)

First discovered in the 1960s, enthusiasm for the benefits of this class of drug resulted in its addiction becoming more difficult to treat and diagnose. This study explores using Hamilton's PRP-1 HPLC column to examine metabolites from benzodiazepine use.

Industrial Applications of HPLC

While dyes are common in providing appeal for foods and beverages, consumer concern about their use is growing. Using reversed-phase HPLC, synthetic dyes can be analyzed and further studied.


Fast HPLC Analysis of Dyes in Foods and Beverages (PRP-C18)

While dyes are common in providing appeal for foods and beverages, consumer concern about their use is growing. Using reversed-phase HPLC, synthetic dyes can be analyzed and further studied.

HPLC Column Cosmetics Applications

Across the globe, many chemicals and products are used for cosmetic applications. Dive deeper into how Hamilton's HPLC applications for cosmetics can help understand the chemicals and compounds in dyes, oils, and more.

Allergens Contained in Balsam of Peru (PRP-C18 / PRP-1)

The balsam of Peru, an essential oil used in perfumes, sunscreens, and beverage flavoring, has been shown to cause allergies. Thanks to Hamilton's PRP-C18 and PRP-1, individual compounds can be isolated for a better understanding of the root cause.

Microlab 600 Application Notes

Hamilton's Microlab 600 diluter dispenser is used across a wide variety of HPLC applications. Learn more about some of the different ways this tool can be utilized.

Milk Protein Analysis by the Amido Black Technique using the Microlab 600 for Sample Preparation

A colorimetric method enables quick determination of protein matter in raw milk samples. Using the Microlab 600 and the Amido Black technique, the total protein level can be found to establish the commercial value of the milk.

Microlab 600 Dilution of Metal Samples for Spectroscopic and Spectrometric Analysis

Atomic Absorption and Inductively Coupled PlasmaOptical Emission Spectroscopy, or Mass Spectrometry, are commonly used for the speciation and determination of metals in environmental samples and consumer products. Microlab 600 provides improved accuracy and precision during these processes.

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