The Power of In-Line Cell Density Measurement
The value of continuous, in-line cell density measurement has been long known in the bioprocessing world as evidenced by the prominence of on-line pH, DO, and similar sensors. Understanding cell growth is possibly the most important parameter of a bioprocess (cell culture or fermentation) and yet is commonly measured at discrete time points only. With Hamilton's selection of cell density sensors, the power of on-line measurement can be utilized for continuous, real-time viable and total cell density analysis.
Typical analysis of viable cell density or cell viability is done through offline sampling and manual or automated analysis. Off-line analysis methods have many deficiencies that lead to large amounts of time and effort and considerable risk and error.
Limitations of Off-line Measurements
Process of Offline Sampling
Time-consuming, requiring skilled labor, risking process contamination
Errors in Offline Counting
Sampling, dilution, and counting errors can lead to process adjustment decisions in the wrong direction.
Missed Process Events
In-line cell density measurement removes the mystery between discrete measuring points, revealing otherwise missed process events like the ideal time for induction, transfer of the starter-culture, or harvesting.
- Filamentous fungi
Compatible Process Types
Incyte and Dencytee are compatible with all typical bioprocessing types.
Arc View Controllers
Hamilton’s specialized Arc View Controllers allow you to visualize and record data from and communicate with Incyte and Dencytee cell density sensors from a single tool. Viable Cell Density (Incyte) and Total Cell Density (Dencytee) data can be viewed on one graph for real-time visualization of cell viability. Hamilton offers several different controller options depending on your needs. See all cell density controllers.
App Note - Online monitoring of CHO cell culture
App Note - Real-time cell density measurement for PAT applicationsAn application highlighting the use of Incyte and Dencytee in a PAT approach to monoclonal antibody (mAb) production.
App Note - Xylitol fermentationLearn how Hamilton pH, DO, and cell density sensor technology helps the Budapest University of Technology and Economics work with different yeast strains to optimize xylitol yield.
App Note - Real-time total cell density measurement of yeast fermentationsDencytee is used to optimize fermentation conditions for yeast processing.
App Note - Real-Time VCD Monitoring of Moss for Therapeutic Protein ProductionIn-line Measurement of Viable Cell Density in a Photobioreactor
Brochure - Cell Density
Support Resource - Incyte Data Analysis MacroImport offline data from the offline data worksheet and Incyte data into this macro for automatic generation of data analysis graphs.
Support Resource - Incyte Protocol for Offline Measurement Correlation (Demo Protocol)This guide details how to obtain the relevant offline measurements and online Incyte (permittivity) data of viable cell density to establish a correlation.
Support Resource - Offline Data Worksheet for IncyteEnter offline viable cell counts or measurements in the offline data worksheet for direct import into the data analysis makro.
Will Incyte work with my cell line?Incyte has been shown to work with cell lines ranging from cell culture to microbial fermentation. We have compiled a current list of known applications in our cell density applications ebook. Download a copy to learn more.
How does Incyte measure viable cell density?Incyte is a capacitance-based sensor responsive only to living cells.
What is the effect of gas bubbles on the Incyte measurement?Gas bubbles have two main impacts: 1. Decrease in the signal intensity, since the Sensor is not measuring in air 2. Increase of background noise
Incyte Measurement Principles
Cell Density Knowledge Base ArticlesA summary page with links for all Cell Density related articles
Connecting Cell Density (Pre-amp Versions) to External SystemsThis article discusses the various options to output cell density measurement data to external devices such as PLCs and control systems.
Data Analysis Tools (Cell Density Pre-Amp versions)Hamilton provides several tools to interpret cell density data and correlate it with offline measurements. Download them here.
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