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HPLC Columns

HPLC column Hamilton

Stationary Phases for Chromatographic Separations

For more than 35 years, Hamilton Company has developed and manufactured pressure-stable, polymeric polystyrene-divinylbenzene (PS-DVB) HPLC columns that are used in most of the world’s top chromatography labs. With a wide range of particle sizes, pore sizes, pH stability from 1 to 14, temperature resistance over 100°C, and chemistries to match most analyte types, Hamilton polymeric columns are the chromatographer's choice for challenging separations.

Product Selector Applications Index

What are HPLC Chromatography Columns?

High-performance liquid chromatography (HPLC), is an analytical technique in chemistry used to separate, purify, identify, and quantify each component in a chemical mixture. In HPLC systems, high-pressure pumps force solvents containing a chemical sample mixture through a metal or polymer plastic tube that is filled with a stationary phase in the form of small, spherical particles (typically less than 100 µm in diameter). Each chemical analyte of the sample mixture interacts differently with the stationary particles and flows through the column at different rates of speed, passing through a detector at the end of the column at different times where they are recorded.

Chromatography columns are most commonly employed in the scientific fields of pharmaceutical, biopharmaceutical, environmental, toxicology, food and beverage, etc. Common uses of HPLC include detecting environmental contaminants in soil and groundwater, production of medications, purification of DNA, etc.

Types of Chromatography Columns

Reversed-Phase

Hamilton reversed-phase chromatography columns combine the best characteristics of silica-based and polymeric columns to arrive at a product that is highly inert and long-lasting.

Anion Exchange

In anion exchange chromatography, the stationary bed has an ionically positive (+) charged surface while the sample ions are of negative (-) charge. The stronger the negative charge on the sample, the stronger it will be attracted to the positive charge on the stationary phase.

Cation Exchange

In cation exchange chromatography, the stationary bed has an ionically negative (-) charged surface while the sample ions are of positive (+) charge. This technique is used almost exclusively with ionic or ionizable samples.

Ion Exclusion

Ion exclusion chromatography is an alternative to ion exchange chromatography in which ionized samples are excluded from the pores of the support and elute first, while the weakly ionized and non-ionic compounds elute later.

Latest Chromatography Application Notes

Separation of Biologically Active Increasing Length Peptides

Biologically active peptides harbor an important role in human health. They are associated with the development of new therapeutic health care products and as potential biologic pathway markers and metabolites in the detection of disease.
Catechins Identified by Hamilton HPLC Column

Spill the Tea, Catechins Identified by Hamilton’s PRP-C18

Catechins found in tea (green, white, black), are best known for their antioxidant properties. Catechins have shown to exhibit antimicrobial activity against staphylococcus, E. Coli, and H. pylori1 in addition to preventing oxidative degradation. Read our app note on catechins identified by Hamilton's PRP-C18.
HPLC chromatography Hamilton

Vitamin E(xtraordinary), Isolation of Tocopherols by PRP-1

Tocopherols are highly important antioxidant vitamins found mainly in the chloroplasts of plants. Read our app note on Vitamin E.


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HPLC Application Index

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USP 'L' Column Listing

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Hamilton Tech Center

Get information on the care and storage of Hamilton HPLC columns, specifications on each column resin, and tips on installing and using Hamilton HPLC guard columns and HPLC guard cartridge systems.

HPLC Column Care & Troubleshooting
HPLC Resin Specifications
HPLC Guard Cartridge Holder Assembly - PEEK
HPLC Guard Cartridge Holder Assembly - Stainless Steel
Safety Data Sheets

Resources

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