HPLC Columns

HPLC Stationary phases for a broad range of separations

For more than 35 years, Hamilton Company has developed and manufactured pressure-stable, polymeric polystyrene-divinylbenzene (PS-DVB) HPLC columns that are used in most of the world’s top chromatography labs. With a wide range of particle sizes, pore sizes, pH stability from 1 to 14, temperature resistance over 100°C, and chemistries to match most analyte types, Hamilton polymeric columns are the chromatographer's choice for challenging separations.

Product Selector Featured App Notes Applications Index

Reversed-Phase

Hamilton reversed-phase HPLC columns combine the best characteristics of silica-based and polymeric columns to arrive at a product that is highly inert and long-lasting.

Anion Exchange

In anion exchange chromatography, the stationary bed has an ionically positive (+) charged surface while the sample ions are of negative (-) charge. The stronger the negative charge on the sample, the stronger it will be attracted to the positive charge on the stationary phase.

Cation Exchange

In cation exchange chromatography, the stationary bed has an ionically negative (-) charged surface while the sample ions are of positive (+) charge. This technique is used almost exclusively with ionic or ionizable samples.

Ion Exclusion

Ion exclusion chromatography is an alternative to ion exchange chromatography in which ionized samples are excluded from the pores of the support and elute first, while the weakly ionized and non-ionic compounds elute later.

Latest Application Notes

Pick Your Poison. Isolation of Paclitaxel. (PRP-1)

The diterpenoid, paclitaxel, which was identified as a potent chemotherapy agent for breast and ovarian cancer originates from the Pacific Yew tree. Though the life-saving compound was initially found in the bark, its presence was later confirmed in every part of the tree excluding the fruit. Paclitaxel, is a member of the taxane family and has been associated with the highly toxic nature of the Taxus genus.

Poisoned by Plastic: The Analysis of BPA and its Derivatives in Water Bottles

Read our app note to investigate whether BPA Free bottles are truly BPA free.

Milk Protein Analysis by the Amido Black Technique using the Microlab 600 for Sample Preparation

Read our app note to find out how to establish the commercial value of milk using the Amido Black Technique on the Microlab 600.

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HPLC Application Index

Find what's possible with a Hamilton HPLC column.

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USP 'L' Column Listing

Search chromatography columns by USP "L" Listing.

Quickly identify the Hamilton HPLC column equivalents to United States Pharmacopeia Convention (USP) listings!

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Tech Center

Get information on the care and storage of Hamilton HPLC columns, specifications on each column resin, and tips on installing and using Hamilton HPLC guard columns and HPLC guard cartridge systems.

HPLC Column Care & Troubleshooting

HPLC Resin Specifications

HPLC Guard Cartridge Holder Assembly - PEEK

HPLC Guard Cartridge Holder Assembly - Stainless Steel

Safety Data Sheets

Hamilton HPLC Column Grants

Hamilton Company proudly supports higher education by awarding one HPLC Column to up to five teaching and research facilities each month. Learn how you can apply today!

University Discount Program

Hamilton is committed to higher education and believes in the importance of developing the next generation of scientists. Learn how you can save 25% on all syringes, needles, and HPLC columns purchased throughout the year.

Additional Resources for HPLC Columns

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Specific Applications

Featured Application

Arsenic Speciation by Anion Exchange HPLC (PRP-X100)

Resolution of arsenic compounds is achieved by anion exchange chromatography on a Hamilton PRP-X100 column.

Resources

Hamilton's knowledge base provides a range of explanatory articles, frequently asked questions, and document downloads.

Featured Download

Reference Guide - HPLC Columns

Learn how to choose a column, how to maximize the life of a column through proper care, cleaning, and restoration.

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How do I restore my HPLC column?

HPLC column chromatography performance will inevitably degrade as the column is used, however this procedure may restore the performance of a column that has become fouled. Click the headline above to read this step-by-step guide.
How Do I Connect a Stainless Steel Guard Cartridge to My HPLC Column?

The process of connecting a guard cartridge to an HPLC column is fairly simple, but requires different steps based on the HPLC instrument and the guard cartridge configuration. Click the headline above to read this step-by-step guide.
How Do I Install My HPLC Column?

Connecting an HPLC column is not difficult, but if the proper steps aren't followed, chromatographic results can be compromised. Click the headline above to read this step-by-step guide.

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SDS

Work with R&D engineers to customize Hamilton parts

Hamilton's "Custom Built" program puts you directly in contact with a Hamilton R&D Engineer to discuss your application and product needs. The engineer will point you to a standard solution or propose a customized solution to meet your needs.

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