Stationary Phases for Chromatographic Separations
For more than 35 years, Hamilton Company has developed and manufactured pressure-stable, polymeric polystyrene-divinylbenzene (PS-DVB) HPLC columns that are used in most of the world’s top chromatography labs. With a wide range of particle sizes, pore sizes, pH stability from 1 to 14, temperature resistance over 100°C, and chemistries to match most analyte types, Hamilton polymeric columns are the chromatographer's choice for challenging separations.
What are HPLC Chromatography Columns?
High-performance liquid chromatography (HPLC), is an analytical technique in chemistry used to separate, purify, identify, and quantify each component in a chemical mixture. In HPLC systems, high-pressure pumps force solvents containing a chemical sample mixture through a metal or polymer plastic tube that is filled with a stationary phase in the form of small, spherical particles (typically less than 100 µm in diameter). Each chemical analyte of the sample mixture interacts differently with the stationary particles and flows through the column at different rates of speed, passing through a detector at the end of the column at different times where they are recorded.
Chromatography columns are most commonly employed in the scientific fields of pharmaceutical, biopharmaceutical, environmental, toxicology, food and beverage, etc. Common uses of HPLC include detecting environmental contaminants in soil and groundwater, production of medications, purification of DNA, etc.
Types of Chromatography Columns
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HPLC Application Index
Find what's possible with a Hamilton HPLC column.
- Enter the compound of interest or search by packing material.
- Narrow your search by detection mode, mobile phase, etc.
- See the method conditions and chromatogram.
Reference Guide - HPLC Columns
How do I restore my HPLC column?Performance will inevitably degrade as the column is used, however this procedure may restore the performance of a column that has become fouled. Learn more in this step-by-step guide.
How Do I Connect a Stainless Steel Guard Cartridge to My HPLC Column?The process of connecting a guard cartridge to an HPLC column is fairly simple, but requires different steps based on the HPLC instrument and the guard cartridge configuration. Click to read our step-by-step guide.
How Do I Install My HPLC Column?Connecting an chromatography column is not difficult, but if the proper steps aren't followed, chromatographic results can be compromised. Follow this step-by-step guide to get it done right.
Work with R&D engineers to customize Hamilton parts
Hamilton's "Custom Built" program puts you directly in contact with a Hamilton R&D Engineer to discuss your application and product needs. The engineer will point you to a standard solution or propose a customized solution to meet your needs.Talk to an engineer