HPLC Columns

HPLC Stationary phases for a broad range of separations
For more than 35 years, Hamilton Company has developed and manufactured pressure-stable, polymeric polystyrene-divinylbenzene (PS-DVB) HPLC columns that are used in most of the world’s top chromatography labs. With a wide range of particle sizes, pore sizes, pH stability from 1 to 14, temperature resistance over 100°C, and chemistries to match most analyte types, Hamilton polymeric columns are the chromatographer's choice for challenging separations.
Reversed-Phase
Anion Exchange
Cation Exchange
Ion Exclusion
Latest Application Notes
Follow Hamilton Lab Products on LinkedIn to receive instant updates on new applications notes
HPLC Application Index
Find what's possible with a Hamilton HPLC column.
- Enter the compound of interest or search by packing material.
- Narrow your search by detection mode, mobile phase, etc.
- See the method conditions and chromatogram.
USP 'L' Column Listing
Search chromatography columns by USP "L" Listing.
Quickly identify the Hamilton HPLC column equivalents to United States Pharmacopeia Convention (USP) listings!
Tech Center
Get information on the care and storage of Hamilton HPLC columns, specifications on each column resin, and tips on installing and using Hamilton HPLC guard columns and HPLC guard cartridge systems.
Additional Resources for HPLC Columns
See Hamilton Laboratory Products
Resources
-
How do I restore my HPLC column?
HPLC column chromatography performance will inevitably degrade as the column is used, however this procedure may restore the performance of a column that has become fouled. Click the headline above to read this step-by-step guide. -
How Do I Connect a Stainless Steel Guard Cartridge to My HPLC Column?
The process of connecting a guard cartridge to an HPLC column is fairly simple, but requires different steps based on the HPLC instrument and the guard cartridge configuration. Click the headline above to read this step-by-step guide. -
How Do I Install My HPLC Column?
Connecting an HPLC column is not difficult, but if the proper steps aren't followed, chromatographic results can be compromised. Click the headline above to read this step-by-step guide.

Work with R&D engineers to customize Hamilton parts
Hamilton's "Custom Built" program puts you directly in contact with a Hamilton R&D Engineer to discuss your application and product needs. The engineer will point you to a standard solution or propose a customized solution to meet your needs.
Talk to an engineer