HPLC Columns

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Hamilton Company has been developing and manufacturing pressure-stable polymeric, high performance liquid chromatography (HPLC) columns for nearly 35 years. We are an established name in science whose products are found in most of the world’s top chromatography labs. From columns to syringes to septa and more, Hamilton Company offers a full line of off-the-shelf and custom chromatography products for HPLC and gas chromatography (GC). Hamilton manufactures stationary phases in a variety of particle sizes and several pore sizes to satisfy surface area requirements. Our columns fit any HPLC system and are designed for reversed-phase chromatography, ion exchange chromatography, and ion exclusion chromatography. Hamilton delivers a wide breadth of products to serve scientific disciplines from research and development, to method development, to scale up chromatography.

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Reversed-Phase

Hamilton reversed-phase HPLC columns combine the best characteristics of silica-based and polymeric columns to arrive at a product that is highly inert and long-lasting.

Anion Exchange

In anion exchange chromatography, the stationary bed has an ionically positive (+) charged surface while the sample ions are of negative (-) charge. The stronger the negative charge on the sample, the stronger it will be attracted to the positive charge on the stationary phase.

Cation Exchange

In cation exchange chromatography, the stationary bed has an ionically negative (-) charged surface while the sample ions are of positive (+) charge. This technique is used almost exclusively with ionic or ionizable samples.

Ion Exclusion

Ion exclusion chromatography is an alternative to ion exchange chromatography in which ionized samples are excluded from the pores of the support and elute first, while the weakly ionized and non-ionic compounds elute later.

HPLC Application Index

Find what's possible with a Hamilton HPLC column.

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See the method conditions and chromatogram.

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USP 'L' Column Listing

Search Chromatography Columns by USP "L" Listing.

Quickly identify the Hamilton HPLC column equivalents to United States Pharmacopeia Convention (USP) listings!

USP L Listings

Tech Center

Get information on the care and storage of Hamilton HPLC columns, specifications on each column resin, and tips on installing and using Hamilton HPLC guard columns.

HPLC Column Care & Storage
HPLC Resin Specifications
HPLC Guard Cartridge Holder Assembly - PEEK
HPLC Guard Cartridge Holder Assembly - Stainless Steel

Resources

Hamilton's knowledge base provides a range of explanatory articles, frequently asked questions, and document downloads.
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Syringe Custom Built

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Hamilton's "Custom Built" program puts you directly in contact with a Hamilton R&D Engineer to discuss your application and product needs. The engineer will point you to a standard solution or propose a customized solution to meet your needs.

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