HPLC Columns
HPLC Stationary phases for a broad range of separations
For more than 35 years, Hamilton Company has developed and manufactured pressure-stable, polymeric polystyrene-divinylbenzene (PS-DVB) HPLC columns that are used in most of the world’s top chromatography labs. With a wide range of particle sizes, pore sizes, pH stability from 1 to 14, temperature resistance over 100°C, and chemistries to match most analyte types, Hamilton polymeric columns are the chromatographer's choice for challenging separations.
Reversed-Phase
Anion Exchange
Cation Exchange
Ion Exclusion
Latest Application Notes
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HPLC Application Index
Find what's possible with a Hamilton HPLC column.
- Enter the compound of interest or search by packing material.
- Narrow your search by detection mode, mobile phase, etc.
- See the method conditions and chromatogram.
USP 'L' Column Listing
Search chromatography columns by USP "L" Listing.
Quickly identify the Hamilton HPLC column equivalents to United States Pharmacopeia Convention (USP) listings!
Apply for an HPLC Column Grant Today!
Hamilton Company proudly supports higher education by awarding one HPLC Column to up to five teaching and research facilities each month. Learn how you can apply today!
Tech Center
Get information on the care and storage of Hamilton HPLC columns, specifications on each column resin, and tips on installing and using Hamilton HPLC guard columns and HPLC guard cartridge systems.
Hamilton Values Higher Education
Hamilton is committed to higher education and believes in the importance of developing the next generation of scientists. Learn how you can save 25% on all syringes, needles, and HPLC columns purchased throughout the year.
Additional Resources for HPLC Columns
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Resources
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How do I restore my HPLC column?
HPLC column chromatography performance will inevitably degrade as the column is used, however this procedure may restore the performance of a column that has become fouled. Click the headline above to read this step-by-step guide. -
How Do I Connect a Stainless Steel Guard Cartridge to My HPLC Column?
The process of connecting a guard cartridge to an HPLC column is fairly simple, but requires different steps based on the HPLC instrument and the guard cartridge configuration. Click the headline above to read this step-by-step guide. -
How Do I Install My HPLC Column?
Connecting an HPLC column is not difficult, but if the proper steps aren't followed, chromatographic results can be compromised. Click the headline above to read this step-by-step guide.
Work with R&D engineers to customize Hamilton parts
Hamilton's "Custom Built" program puts you directly in contact with a Hamilton R&D Engineer to discuss your application and product needs. The engineer will point you to a standard solution or propose a customized solution to meet your needs.
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