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Stationary Phases for Chromatographic Separations

For more than 35 years, Hamilton Company has developed and manufactured pressure-stable, polymeric polystyrene-divinylbenzene (PS-DVB) HPLC columns that are used in most of the world’s top chromatography labs. With a wide range of particle sizes, pore sizes, pH stability from 1 to 14, temperature resistance over 100°C, and chemistries to match most analyte types, Hamilton polymeric columns are the chromatographer's choice for challenging separations.

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What are HPLC Chromatography Columns?

High-performance liquid chromatography (HPLC), is an analytical technique in chemistry used to separate, purify, identify, and quantify each component in a chemical mixture. In HPLC systems, high-pressure pumps force solvents containing a chemical sample mixture through a metal or polymer plastic tube that is filled with a stationary phase in the form of small, spherical particles (typically less than 100 µm in diameter). Each chemical analyte of the sample mixture interacts differently with the stationary particles and flows through the column at different rates of speed, passing through a detector at the end of the column at different times where they are recorded.

Chromatography columns are most commonly employed in the scientific fields of pharmaceutical, biopharmaceutical, environmental, toxicology, food and beverage, etc. Common uses of HPLC include detecting environmental contaminants in soil and groundwater, production of medications, purification of DNA, etc.

Types of Chromatography Columns

Latest Chromatography Application Notes

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HPLC Application Index

Find what's possible with a Hamilton HPLC column.

  1. Enter the compound of interest or search by packing material.
  2. Narrow your search by detection mode, mobile phase, etc.
  3. See the method conditions and chromatogram.


USP 'L' Column Listing

Search chromatography columns by USP "L" Listing.

Quickly identify the Hamilton HPLC column equivalents to United States Pharmacopeia Convention (USP) listings!


Hamilton Tech Center

Get information on the care and storage of Hamilton HPLC columns, specifications on each column resin, and tips on installing and using Hamilton HPLC guard columns and guard cartridge systems.


Hamilton's knowledge base provides a range of explanatory articles, frequently asked questions, and document downloads.
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