HPLC Columns

laboratory solutions

Stationary Phases for Chromatographic Separations

For more than 35 years, Hamilton Company has developed and manufactured pressure-stable, polymeric polystyrene-divinylbenzene (PS-DVB) HPLC columns that are used in most of the world’s top chromatography labs. With a wide range of particle sizes, pore sizes, pH stability from 1 to 14, temperature resistance over 100°C, and chemistries to match most analyte types, Hamilton polymeric columns are the chromatographer's choice for challenging separations.

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What are HPLC Chromatography Columns?

High-performance liquid chromatography (HPLC), is an analytical technique in chemistry used to separate, purify, identify, and quantify each component in a chemical mixture. In HPLC systems, high-pressure pumps force solvents containing a chemical sample mixture through a metal or polymer plastic tube that is filled with a stationary phase in the form of small, spherical particles (typically less than 100 µm in diameter). Each chemical analyte of the sample mixture interacts differently with the stationary particles and flows through the column at different rates of speed, passing through a detector at the end of the column at different times where they are recorded.

Chromatography columns are most commonly employed in the scientific fields of pharmaceutical, biopharmaceutical, environmental, toxicology, food and beverage, etc. Common uses of HPLC include detecting environmental contaminants in soil and groundwater, production of medications, purification of DNA, etc.

Types of Chromatography Columns

Reversed-Phase

Hamilton reversed-phase chromatography columns combine the best characteristics of silica-based and polymeric columns to arrive at a product that is highly inert and long-lasting.

Anion Exchange

In anion exchange chromatography, the stationary bed has an ionically positive (+) charged surface while the sample ions are of negative (-) charge. The stronger the negative charge on the sample, the stronger it will be attracted to the positive charge on the stationary phase.

Cation Exchange

In cation exchange chromatography, the stationary bed has an ionically negative (-) charged surface while the sample ions are of positive (+) charge. This technique is used almost exclusively with ionic or ionizable samples.

Ion Exclusion

Ion exclusion chromatography is an alternative to ion exchange chromatography in which ionized samples are excluded from the pores of the support and elute first, while the weakly ionized and non-ionic compounds elute later.

Build Your Own HPLC Column

If you can't find the Hamilton column you need, we can make it for you. Choose your desired Hamilton stationary phase and the column hardware for your application needs and we'll make it!

Custom HPLC Column Packing Services

Trust Hamilton's experienced chromatography team to expertly produce your analytical-to-preparative scale HPLC columns with your own stationary phase.

Latest Chromatography Application Notes

App Note: Oasis or Sand Dune? Isolation of Psychedelic Compounds

Oasis or Sand Dune? Isolation of Psychedelic Compounds

Magic mushrooms have recently seen a resurgence in popularity due to new findings regarding the effects of psilocybin and psilocin on serotonin receptors. Hamilton Company has developed a method for isolating and identifying five common psychedelic compounds using an alkaline ion-pairing agent, hexylamine, which shows good peak shape and resolution.
GPCR App Note - The Biological Traffic Modulator

GPCRs - The Biological Traffic Modulator

The analysis of peptides in the GI tract has revealed connections between these peptides and other body systems, such as the endocrine, cardiac, and central nervous systems. Understanding the relationship between these peptides is essential to understanding the body's balance. Hamilton Company's HPLC stationary phases are critical to isolating GPCR binding peptides.

Separation of Biologically Active Increasing Length Peptides

Biologically active peptides harbor an important role in human health. They are associated with the development of new therapeutic health care products and as potential biologic pathway markers and metabolites in the detection of disease.


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USP 'L' Column Listing

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Hamilton Tech Center

Get information on the care and storage of Hamilton HPLC columns, specifications on each column resin, and tips on installing and using Hamilton HPLC guard columns and guard cartridge systems.

HPLC Column Care & Troubleshooting
HPLC Resin Specifications
HPLC Guard Cartridge Holder Assembly - PEEK
HPLC Guard Cartridge Holder Assembly - Stainless Steel
Safety Data Sheets

Resources

Hamilton's knowledge base provides a range of explanatory articles, frequently asked questions, and document downloads.
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