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In anion exchange chromatography, the stationary bed has an ionically positive (+) charged surface while the sample ions are of negative (-) charge. This technique is used almost exclusively with ionic or ionizable samples. The stronger the negative charge on the sample, the stronger it will be attracted to the positive charge on the stationary phase, and thus the longer it will take to elute. Elution in ion chromatography is effected by mobile phase pH and ionic-strength, and, to a lesser extent, operation temperature. The ability to use the full pH range and elevated temperatures are distinct advantages compared to silica-based supports.

Anion Exchange Capacity
Anion exchange capacity is a measurement of the number of negative charges (anions) that the exchange resin can bind to and is reported in singly charged ion equivalents per 1 gram of resin. Exchange capacity is dependent upon the pH of the mobile phase and in anion exchange chromatography; as mobile phase acidity decreases (pH increases), the exchange capacity decreases.

Hamilton offers six polymeric packing materials for anion exchange separations.

Browse Anion Exchange

Prp X100

PRP-X100: Anion Exchange HPLC Column

High capacity ion exchange chromatography of organic and inorganic anions, organic acids, organic and inorganic arsenic species, halides, nucleotides.
Prp X110

PRP-X110: Anion Exchange Chromatography Column

High sensitivity detection ion exchange chromatography of organic and inorganic anions, organic acids, organic and inorganic arsenic species, halides, nucleotides.
Prp X500

PRP-X500: Methacrylate (SAX) Anion Exchange HPLC Column

Superficially porous, polymeric anion exchange coating for strong anion exchange (SAX) purification of proteins, peptides, and DNA/RNA.
Prp X600

PRP-X600: Methacrylate (WAX) Anion Exchange HPLC Column

Superficially porous, polymeric anion exchange coating for weak anion exchange (WAX) purification of nucleic acids such as single stranded/double stranded RNA and DNA, peptides, and proteins.
Rcx 10

RCX-10: Anion Exchange HPLC Columns

High exchange capacity anion exchange column for carbohydrate separation: Carbohydrates, polysaccharides, sugar oligomers up to DP8.
Rcx 30

RCX-30: High Anion Exchange HPLC Columns

Increased exchange capacity anion exchange columns for better separation of carbohydrate mixtures: Complex mono- and disaccharide separation.

Resources

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