BIOSPOT DX platform on a laboratory bench.

BIOSPOT® DX

Precision dispensing
Any substrate
Flexible & precise
Multi-volume control
Pico to microliter
Drop-on-demand
Custom patterns
Camera-guided UI
Point & click setup

Accelerate Innovation

BIOSPOT® DX delivers camera-guided, ultra-low volume dispensing for diagnostics and research. From biosensors to microfluidics, it enables precise droplet placement on diverse substrates, automates workflows with speed and accuracy, and supports confident innovation.

BIOSPOT DX

Versatile, substrate-agnostic dispensing


BIOSPOT DX is built for scientists and diagnostic developers who require precise drop-on-demand liquid control across a wide range of substrates—not just microplates. It dispenses uniform droplets on glass, nitrocellulose, polymers, and hybrid materials, supporting applications like biosensors, lateral flow devices, nucleic acid assays, and point-of-care testing (POCT). Real-time visualization and pattern customization allow for precise alignment and reproducible results.

Volume flexibility and dispensing control


Supporting volumes from picoliters to microliters, BIOSPOT DX integrates PIPEJET® for nanoliter, SIJET for picoliter, and VALVEJET for microliter dispensing. Whether you're printing single drops, drop-on-drop stacks, lines, grids, or complex shapes, the intuitive software makes patterns repeatable and protocol design easy. This flexibility makes it an ideal tool for preparing diagnostic formats with precision and speed.

Camera-guided automation with scalable design


TOPVIEW substrate detection and fiducial recognition ensure automated pattern alignment, scaling, and rotation, while click-to-spot functionality offers intuitive manual control. Whether you're preparing POCT strips, molecular diagnostic slides, or printing capture reagents, BIOSPOT® DX adapts to your workflow with modular decks and customizable configurations—ready to scale from research to production.

Common applications Manufacturing, Point of Care Tests

Featured Technologies
coating of microneedles on transdermal patches
a nanoliter droplet capture in flight
lateral flow printing
a screenshot of the BIOSPOT software
a view of a manufacturing envrironment

Ensure reproducible test results with precision droplet placement for consistent patterning in diagnostics and biosensor workflows.

Hamilton application note cover: "Non-contact microneedle coating utilizing drop-on-demand PIPEJET® technology"
Non-contact microneedle coating utilizing drop-on-demand PIPEJET technology
Materials

SMARTDROP calibrates droplet volume in real time, preventing satellite formation and ensuring consistent, high-quality results across experiments.

Easily work with diverse substrates—slides, membranes, or chips—without changing systems or compromising dispensing quality.

Hamilton BIOSPOT DX white paper cover: "Line-based and array-based lateral flow assay development and production on the BIOSPOT® DX"
Line-based and array-based lateral flow assay development and production on the BIOSPOT® DX
Materials

Create complex dispensing layouts with ease using camera-guided setup, fiducial alignment, and  click-and-dispense patterning.

Modular deck designs, multi-volume integration, and software control allow BIOSPOT DX to grow with your project, from early R&D to full-scale production.

  • BIOSPOT® DX dispensing head with PIPEJET®, and compatible with SIJET, and VALVEJET modules for picoliter to microliter drop-on-demand dispensing.
  • SMARTDROP optical system performing droplet volume calibration and verification in real time.
  • BIOSPOT DX and localization of Live camera view from TOPVIEW showing substrate alignment with fiducial detection and click-to-spot targeting.
  • Open-format BIOSPOT DX deck displaying flexible layout for various substrates including slides, chips, and membranes.
  • Front-facing touchscreen interface on BIOSPOT DX used to access, program, and run batch dispensing protocols.

Intelligent Spotting Platform

The BIOSPOT DX is a modular, camera-guided liquid dispensing system designed for biosensor production, lateral flow assay development, and point-of-care testing workflows. It enables precise drop-on-demand dispensing across a range of volumes and substrates, supporting applications from early-stage research to diagnostic manufacturing. With software-driven control and real-time substrate visualization, BIOSPOT DX offers high reproducibility, flexibility, and speed, all in one compact platform.

1

Multi-Volume Dispensing Flexibility

Enables contact-free dispensing from picoliter to nanoliter volumes using PIPEJET® and SIJET. Perfect for antibodies, enzymes, or capture reagents in diagnostic assay development.
2

SMARTDROP® System

Calibrates droplet size and volume in real time. Ensures reproducible dispensing across substrates and liquids, improving diagnostic workflows and assay accuracy.
3

TOPVIEW Substrate Camera

Provides real-time visualization, fiducial detection, and click-to-spot control. Enables fast, precise droplet placement on dot arrays or custom diagnostic geometries.
4

Supports Any Substrate Format

The open 251 × 380 mm deck supports cartridges, slides, membranes, and microfluidic devices—offering unmatched flexibility beyond microplate-based formats.
5

Touchscreen Workflow Access

Access, create, and save batch protocols directly on the built-in touchscreen—no external PC needed. Simplifies daily operation for fast, autonomous method execution.

TOPVIEW: Live Substrate Visualization and Post-Deposition Quality Control

See how TOPVIEW supports quality control by visualizing the substrate in real time, enabling precise pattern design and verifying droplet deposition after dispensing for confident, reproducible results.

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  • The BIOSPOT: A Trusted Tool Behind Our Upcoming Publications

    The BIOSPOT has been essential to our research, enabling precise, reproducible dispensing across multiple experimental lines—from antimicrobial resistance protein microarrays to QCM sensor calibration. Its performance has directly contributed to high-quality results that are now being prepared for publication.

     

    Dr. Augusto Juste Dolz
    Centro de Investigación e Innovación en Bioingeniería (Ci2B) ,Laboratorio de Inmunotecnología, Universidad Politécnica de Valencia, Spain

BIOSPOT® DX Specifications

BIOSPOT® DX Specifications

Print

System Dimensions

Height
50 cm (1 ft, 7.69 in)
Width
58 cm (1 ft, 10.83 in)
Depth
90 cm (2 ft, 11.43 in)

Dispensing Configuration

PIPEJET
up to 12
SIJET
up to 12
VALVEJET
up to 4
Maximum Supported Configuration
12
Channel Spacing
Customizable

Deck Configuration & Capacity

Addressable Area
251 x 380 mm
Aspiration from Source Plate 1
Configurationd-dependent

Process Control

SMARTDROP
Optional
TOPVIEW
Included
Ionizer
Optional

Positional Accuracy & Movement

Absolute Positioning Accuracy
50 µm
Precision / Step Width
10 µm
Axis Speed
X/Y: 250 mm/s ; Z: 30 mm/s

Control & Software Environment

Computer
Integrated
Software
BFX Control

1 Aspiration from source plate; also supports dispensing pipe cleaning when enabled.

BIOSPOT® DX Resources

Browse app notes, user guides, specification documents, and more in our Knowledge Center.

Browse app notes, user guides, specification documents, and more in our Knowledge Center.

Browse app notes, user guides, specification documents, and more in our Knowledge Center.

Icon of a Polaroid-style camera labeled "DX" on a white circle background

FAQ

Which dispensing technologies are available in BIOSPOT DX?

You can integrate PIPEJET (nL), SIJET (pL), or VALVEJET (µL) depending on your volume and liquid requirements.
Icon of a Polaroid-style camera labeled "DX" on a white circle background

FAQ

How customizable are the dispensing routines in BIOSPOT DX?

The software enables complete protocol programming, including pattern, volume, and liquid handling routines.
Icon of a Polaroid-style camera labeled "DX" on a white circle background

FAQ

What is the SMARTRESERVOIR and how does it improve dispensing?

SmartReservoir is a pressure-controlled module that increases PIPEJET capacity to 13 mL and ensures consistent droplet formation for long, stable dispensing runs with challenging liquids.

Browse app notes, user guides, specification documents, and more in our Knowledge Center.

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Webinar

Precision in Every Drop: Explore New Possibilities in Ultra-Low Volume Dispensing with BIOSPOT

Join Hamilton experts Max Ranall and Thomas Clapes as they cover the ultra-low volume dispense capabilities of Hamilton’s innovative BIOSPOT platform.
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Services
Application and Technical Support

Hamilton's Technical Support team is here to provide product trouble-shooting, repair, application support, virtual start-up support, and more.

Installation Support

Installation, set-up, and calibration support directly on-site. Our field service team ensures your sensors and cables are installed and functioning correctly.

Maintenance and Calibration Services

Preventative maintenance and regular service contracts. Schedule on-site service dates to ensure your sensors are properly maintained and your process is optimized.

Dispensing Services

Hamilton's Dispensing Service offers feasibility testing to evaluate if your liquid and workflow are compatible with our dispensing technology.

Backed by world-class service and support.

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