The analysis of peptides in the GI tract has led to important discoveries about their role in other functional systems. Understanding how these peptides are linked is crucial to understanding the body's equilibrium. Hamilton Company's HPLC stationary phases are essential in isolating GPCR binding peptides, which have shown significance in developing new therapeutic treatments for diseases related to dysfunction in the pathway.

The analysis of the seven peptides highlights the benefits of using the polymer-based PRP-3, a reversed-phase resin. The time to perform the analysis was shortened dramatically due the use of elevated flow rates, allowing for quicker re-equilibration and sharper analyte peaks. Similarly, the covalent bonds found in the PRP-3 exhibit advantageous interactions between the biological π bonds found in the peptides and the available aromatic benzyl rings of the resin. The highly hydrophobic resin made of crosslinked divinylbenzene rings is nearly impervious to either overtly alkaline or acidic mobile phases allowing for great reproducibility between batches and multiple runs.

Author: Adam L. Moore, PhD, Hamilton Company

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Using HPLC to identify GPCR