PRP-3 particles come with a larger (300 Å) pore size for protein separation. No silanol groups on the resin means no irreversible protein adsorption, giving > 90% analyte recovery. This stable material also allows the use of concentrated acids, chaotropes, and even detergents to minimize problems with protein solubility.
Application Examples: Organic compounds: large molecules (> 2,000 mw), peptides, proteins, protein digests, protected and deprotected oligonucleotides, nucleic acids.
PRP-3 Guard Columns
PRP-3 Bulk Resin
The PRP-3 is a reversed-phase column optimized for separation of macromolecules such as DNA, RNA oligos, proteins, peptides and proteomics. Hamilton’s PRP-3 is a polymeric reversed-phase HPLC column designed for the purification and isolation of proteins and peptides with very good recovery (> 90%). It is based off of the PRP-1 but utilizes a 300 Å pore size rather than the PRP-1’s 100 Å pore size. The highly inert polymeric packing poly(styrene-divinylbenzene) enhances protein recovery because there are no silanol groups on the support to cause irreversible protein adsorption.
PRP-3 is a PS-DVB support that is pressure stable up to 5,000 psi and cross-linked to prevent shrinking or swelling when the mobile phase is changed. Chemically, proteins present solubility problems unlike many small molecules. Most proteins are hydrophobic on the inside, with highly charged exteriors. This often presents dissolution problems, particularly when pH is near the isoelectric point of the protein. The rugged chemical nature of the PRP-3 allows the protein chemist a much broader selection of agents for dissolution, including concentrated acids, aggressive chaotropes, as well as detergents. In proteomics, the PRP-3 has excellent potential for single-column 2D HPLC. The orthogonal selectivity between low and high pH separations is often comparable to that achieved with two different column formats (SCX, RP), but with the added bonus of MS-compatible mobile phase.
PRP-3 stationary phase structure and applications
Organic compounds: large molecules (> 2,000 mw), peptides, proteins, protein digests, protected and de-protected oligonucleotides, nucleic acids
Examples of analytes that can be separated on PRP-3 columns:
Synthetic high mw polymers
How to Choose Column Specifications for Your Application
Reference Guide - HPLC ColumnsLearn how to choose a column, how to maximize the life of a column through proper care, cleaning, and restoration.
How do I restore my HPLC column?Performance will inevitably degrade as the column is used, however this procedure may restore the performance of a column that has become fouled. Learn more in this step-by-step guide.
How Do I Connect a Stainless Steel Guard Cartridge to My HPLC Column?The process of connecting a guard cartridge to an HPLC column is fairly simple, but requires different steps based on the HPLC instrument and the guard cartridge configuration. Click to read our step-by-step guide.
How Do I Install My HPLC Column?Connecting an chromatography column is not difficult, but if the proper steps aren't followed, chromatographic results can be compromised. Follow this step-by-step guide to get it done right.
USP "L" Column ListingsExplore Hamilton’s qualifying USP “L” columns.
HPLC Guard Columns, HPLC Guard CartridgesHPLC guard columns and guard cartridges protect analytical, semi-prep and preparative HPLC columns. They help by removing particulate contaminants and highly absorptive compounds from samples, prolonging column life.
Work with R&D engineers to customize Hamilton parts
Hamilton's "Custom Built" program puts you directly in contact with a Hamilton R&D Engineer to discuss your application and product needs. The engineer will point you to a standard solution or propose a customized solution to meet your needs.Talk to an engineer