Build Your Own HPLC Column

Hamilton offers 21 polymer-based stationary phases as well as two silica gels (C8 and C18) to satisfy most separation/purification needs. Our specialty resins are offered in a wide variety of hardware dimensions, and while these sizes cover many applications in varying science focuses, you may need a specific Hamilton resin in a column size we don't offer as off-the-shelf. Hamilton gives you control to build any column to your specifications with any of our stationary phases in any combination of our column hardware formats.
Create The Hamilton HPLC Column that Fits Your Application Needs
Click the appropriate part number below to configure and purchase your column!
HPLC Column Scale | Inner Diameter | Length | Part Number |
---|---|---|---|
Microbore | 1.0 mm - 2.1 mm | 50 mm, 100 mm, 150 mm, 250 mm | 79641 |
Analytical | 4.1 mm - 4.6 mm | 50 mm, 100 mm, 150 mm, 250 mm | 79641 |
Semipreparative | 7.0 mm - 10 mm | 50 mm, 100 mm, 150 mm, 250 mm, 300 mm* | 79642 |
Preparative | 21.2 mm - 30 mm | 50 mm, 100 mm, 150 mm, 250 mm | 79643 |
Preparative | 50 mm | 50 mm, 100 mm, 150 mm, 250 mm | 79644 |
Preparative | 100 mm | 50 mm, 100 mm, 150 mm, 250 mm | 79645 |
*Available for 7.0 mm and 7.8 mm ID hardware
Need Help Deciding Which Hamilton HPLC Column Is Right For You?
1. Choose your stationary phase
If you need additional information to determine which chemistry fits your application needs, check out our application index with over 1,700 compounds separated.
Search applications by stationary phase or compound of interest
Separation Mechanism
We specialize in polymer stationary phases and offer silica stationary phases covering the following separation mechanisms
Reversed-Phase | Anion Exchange |
Cation Exchange | Ion Exclusion |
USP "L" Considerations
If you need to follow a specific USP monograph, browse our USP qualified stationary phases.
2. Select the particle size and hardware dimensions
Choosing HPLC Column Specifications
3. We Build It!
We can repack your Hamilton HPLC column to perform like new using your existing column hardware

Need to Have Your Custom Hamilton HPLC Column Repacked?
Hamilton offers repacking services for your custom Hamilton HPLC column
Here's How It Works
1. Place an Order For The Needed Hardware Size/Scale (above)
2. Send Your HPLC Column Back To Hamilton
We'll work with you to ship your used HPLC column back to us using a traceable shipping method.
Fill out the RMA Form here and we'll get the process started.
3. Hamilton's Experts Will Take Care of The Rest!

Work with R&D engineers to customize Hamilton parts
Hamilton's "Custom Built" program puts you directly in contact with a Hamilton R&D Engineer to discuss your application and product needs. The engineer will point you to a standard solution or propose a customized solution to meet your needs.
Talk to an engineer