Ion exclusion chromatography is an alternative to ion exchange chromatography in which ionized samples are excluded from the pores of the support and elute first, while the weakly ionized and non-ionic compounds elute later. Mixtures of weak acids, like those in fruits and milk products, are frequently not very well separated by pure ion-exchange methods, nor in the reversed-phase mode.
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Reference Guide - HPLC ColumnsLearn how to choose a column, how to maximize the life of a column through proper care, cleaning, and restoration.
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