PRP-X300

Hamilton PRP-X300 columns offer an easy, rapid way to separate closely related alcohols and organic acids. The sulfonated poly(styrenedivinylbenzene) support separates samples via a mixed mode mechanism. Separation on the PRP-X300 is accomplished by three modes: hydrogen bonding, reversed-phase, and ion exclusion. PRP-X300 column selectivity can be altered by changing the pH of the buffer or adding an organic modifier (e.g., methanol, acetonitrile). The support’s stability to organic solvents makes it possible to analyze samples that are too highly retained on conventional ion exclusion supports.
Application Examples: Organic acids and alcohols
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PRP-X300 Columns
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PRP-X300 Guard Columns
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PRP-X300 Bulk Resin
PRP-X300 Overview
Hamilton PRP-X300 columns offer an easy, rapid way to separate closely related alcohols and organic acids. The sulfonated poly(styrenedivinylbenzene) support separates samples via a mixed mode mechanism. Separation on the PRP-X300 is accomplished by three modes: hydrogen bonding, reversed-phase, and ion exclusion. PRP-X300 column selectivity can be altered by changing the pH of the buffer or adding an organic modifier (e.g., methanol, acetonitrile). The support’s stability to organic solvents makes it possible to analyze samples that are too highly retained on conventional ion exclusion supports. The high performance packing ensures narrow peaks under isocratic conditions. The use of isocratic conditions allows samples to be analyzed one after another without waiting for column re-equilibration. Most samples require only minimal preparation before injection, increasing sample throughput in the lab.
How to Choose Column Specifications for Your Application
Tech Center
Specific Applications
Resources
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Reference Guide - HPLC Columns
Learn how to choose a column, how to maximize the life of a column through proper care, cleaning, and restoration.
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How Do I Connect a PEEK Guard Cartridge to My HPLC Column?
The process of connecting a guard cartridge to a HPLC column is straightforward, but requires different steps based on the HPLC instrument and its configuration. Click the headline above to read this step-by-step guide. -
How Do I Connect a Stainless Steel Guard Column to My HPLC Column?
Connecting a guard column to an HPLC column is straightforward, but requires different steps based on the HPLC instrument and its configuration. Click the headline above to read this step-by-step guide.
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USP "L" Column Listings
Explore Hamilton’s qualifying USP “L” columns. -
HPLC Guard Columns, HPLC Guard Cartridges
HPLC guard columns and guard cartridges protect analytical, semi-prep and preparative HPLC columns. They help by removing particulate contaminants and highly absorptive compounds from samples, prolonging column life.

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