PRP-X300

Prp X300

Hamilton PRP-X300 columns offer an easy, rapid way to separate closely related alcohols and organic acids. The sulfonated poly(styrenedivinylbenzene) support separates samples via a mixed-mode mechanism. Separation on the PRP-X300 is accomplished by three modes: hydrogen bonding, reversed-phase, and ion exclusion. PRP-X300 column selectivity can be altered by changing the pH of the buffer or adding an organic modifier (e.g., methanol, acetonitrile). The support’s stability to organic solvents makes it possible to analyze samples that are too highly retained on conventional ion exclusion supports.
 

Application Examples: Organic acids and alcohols

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PRP-X300 Columns

Hamilton PRP-X300 columns offer a quick way to separate closely related alcohols and organic acids.
PRP-X300 Columns

PRP-X300 Guard Columns

Protect your PRP-X300 column.
PRP-X300 Guard Columns

PRP-X300 Bulk Resin

Pack your own column.
PRP-X300 Bulk Resin

PRP-X300 Overview

Hamilton PRP-X300 columns offer an easy, rapid way to separate closely related alcohols and organic acids. The sulfonated poly(styrenedivinylbenzene) support separates samples via a mixed mode mechanism. Separation on the PRP-X300 is accomplished by three modes: hydrogen bonding, reversed-phase, and ion exclusion. PRP-X300 column selectivity can be altered by changing the pH of the buffer or adding an organic modifier (e.g., methanol, acetonitrile). The support’s stability to organic solvents makes it possible to analyze samples that are too highly retained on conventional ion exclusion supports. The high performance packing ensures narrow peaks under isocratic conditions. The use of isocratic conditions allows samples to be analyzed one after another without waiting for column re-equilibration. Most samples require only minimal preparation before injection, increasing sample throughput in the lab.

Tech Center

HPLC Column Care & Troubleshooting
HPLC Resin Specifications
HPLC Guard Cartridge Holder Assembly - PEEK
HPLC Guard Cartridge Holder Assembly - Stainless Steel
Safety Data Sheets
Choosing Column Specifications

PRP-X300 Resources

Browse app notes, user guides, specification documents, and more in our Knowledge Center.

Browse app notes, user guides, specification documents, and more in our Knowledge Center.

HAM SDS PRP X300 resin dry

SDS

SDS - PRP-X300 (resin, dry)

Download the SDS for Hamilton PRP – X300 Resin (Dry), an HPLC filter media. Review important safety information including handling guidelines, health hazards, and first aid procedures.
Separation of Halo Acetic Acids

Application Note

Separation of Halo Acetic Acids by Ion Exclusion (PRP-X300)

In this app note. we explore a method that isolates fluoro, chloro, bromo, and iodoacetic acids from water samples which highlights the effectiveness and robustness of the Hamilton PRP-X300.
HAM Reference Guide HPLC Columns

Guide

Reference Guide - HPLC Columns

Learn how to choose a column, how to maximize the life of a column through proper care, cleaning, and restoration.

Browse app notes, user guides, specification documents, and more in our Knowledge Center.

cation exchange column

Article

USP "L" Column Listings

Explore Hamilton’s qualifying USP “L” columns.

Browse app notes, user guides, specification documents, and more in our Knowledge Center.

HPLC FAQ

FAQ

How Do I Connect a Stainless Steel Guard Column to My HPLC Column?

Connecting a guard column to an HPLC column is straightforward, but requires different steps based on the HPLC instrument and its configuration. Click the headline above to read this step-by-step guide.
HPLC FAQ

FAQ

How Do I Connect a PEEK Guard Cartridge to my HPLC Column?

The process of connecting a guard cartridge to a HPLC column is straightforward, but requires different steps based on the HPLC instrument and its configuration. Click the headline above to read this step-by-step guide.
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