Dual Frequency Mode
Dual frequency mode measures at a cell type specific frequency (fmes). This frequency is usually 1 MHz for mammalian cells and bacteria, and 2 MHz for yeast. It is continuously and automatically corrected for the background permittivity measured at high frequency (fhigh). The permittivity measured by Incyte is specific to viable cells and correlates with the viable cell density, specially during the exponential growth phase.
Frequency Scan Mode
As a research tool it is possible to run a frequency scanning.
The polarization behavior of cells varies strongly at different frequencies. Cells fully polarize at low frequency whereas they hardly polarize at high frequency. The Incyte Scan measures the permittivity signal at 17 different frequencies between 0.3 and 10 MHz
and provides additional information especially on the cells physiology.
During the Incyte Scan the most relevant parameters - Δεmax, fc, and α - are automatically calculated and displayed.
- The characteristic frequency, fc, may provide an indication of the average cell diameter.
- The height of the low frequency plateau, Δεmax, may better correlate with the viable cell density at specific applications
- The slope (α) of the beta-dispersion at the characteristic frequency fc may provide an indication of the distribution of the cell diameter.