Hyaluronic acids (HA) are anionic, non-sulfated glycosaminoglycans distributed widely throughout connective, epithelial and neural tissues. Due to its biocompatibility, HA are frequently used in many medical applications and the cosmetic industry. HA may consist of very large molecules with molecular weight ranging several million grams per mol (g/mol), which makes solutions very viscous and difficult to handle. Traditional air-displacement technologies, such as pipettes, often fail or deliver inaccurate values due to the error in the measured volume caused by the sample’s density and viscosity. Positive displacement of viscous solutions is commonly used with manual syringes which offer excellent accuracy but less reproducibility due to manual handling. For this reason, the Microlab 600 was used for handling of HA solutions since the instrument combines the unique accuracy and precision of Hamilton syringes with the outstanding reproducibility and user-independent liquid handling capabilities of a semi-automated workstation.
In this application, the Advanced Microlab 600 Dual Syringe Diluter (ML625-DIL) was used for preparation of HA hydrogels for tissue engineering. Tissue engineering is a technology for artificial production of biological tissues by the directed culturing of cells on a biocompatible support material in order to replace or regenerate diseased tissue in a patient.