Incyte (Pre-Amp Version)
The Original Viable Cell Density Sensor
Incyte is a permittivity sensor whose reading can be correlated to the viable cell density. This sensor has been proven across many cell lines and microorganisms: from mammalian and insect cell culture to high density bacterial, yeast or filamentous fungi fermentation. Check out the Cell Density Applications eBook for real data. This real-time sensor can be used directly in the cell culture process, or fermentation for real-time process control, eliminating the need for offline sampling and analysis.
How does Incyte work?
Incyte applies an alternating electric field to the culture and measures the resulting polarization and depolarization of living cells and microorganisms through a permittivity reading (capacitance per area). This signal can be correlated to the viable cell density, because only viable cells can be polarized. Dead cells have a leaky membrane and cannot be polarized. This method is therefore insensitive to dead cells, cell debris, and microcarriers.
Accessories for Incyte Sensors
A measuring loops consist of an Incyte sensor and a pre-amplifier, which are factory calibrated. The Sensor Unit is connected to a Cell Density Controller and provides a variety of communication protocols, from analog 4-20 mA signal to digital OPC-XML-DA connection. Diverse accessories are available to complete the portfolio.
Cell Density Controllers and Accessories
Cell Density Cables
Cell Density Maintenance
Specific Applications
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App Note - Real-time cell density measurement for PAT applications
An application highlighting the use of Incyte and Dencytee in a PAT approach to monoclonal antibody (mAb) production. -
App Note - Online monitoring of CHO cell culture
A suite of Hamilton sensors is used to continuously monitor batch fermentation of CHO cells for tighter process control.
Resources
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Brochure - BioPharma
Innovative solutions for process analytics in biotechnological and pharmaceutical industries. -
Infographic - Online Cell Density
An infographic highlighting the use and benefits of on-line cell density measurement. -
Quick Guide - Validation / Calibration Incyte Sensors (Pre-amp Version)
Quick Guide for Validation / Calibration Incyte (Pre-amp Version)
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How often do Incyte sensors (pre-amp version) need to be calibrated?
Hamilton recommends once per year with a validation at 6 months. -
How does Incyte measure viable cell density?
Incyte is a capacitance-based sensor responsive only to living cells. -
What is A-length and how is it measured?
A-length refers to the insertion depth of the sensor. It's generally measured from the base of the PG13.5 mounting threads but can vary with certain styles of sensors.
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Connecting Cell Density (Pre-amp Versions) to External Systems
This article discusses the various options to output cell density measurement data to external devices such as PLCs and control systems. -
Data Analysis Tools (Cell Density Pre-Amp versions)
Hamilton provides several tools to interpret cell density data and correlate it with offline measurements. Download them here. -
Incyte Sensors (Pre-amp Version) - Standard vs. Low Conductivity
There are several different options for Incyte cell density sensors based on liquid conductivity. Learn more about sensor selection / applications.
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