Incyte (Pre-Amp Version)
The Original Viable Cell Density Sensor
Incyte is a permittivity sensor whose reading can be correlated to the viable cell density. This sensor has been proven across many cell lines and microorganisms: from mammalian and insect cell culture to high density bacterial, yeast or filamentous fungi fermentation. Check out the Cell Density Applications eBook for real data. This real-time sensor can be used directly in the cell culture process, or fermentation for real-time process control, eliminating the need for offline sampling and analysis.
How does Incyte work?
Incyte applies an alternating electric field to the culture and measures the resulting polarization and depolarization of living cells and microorganisms through a permittivity reading (capacitance per area). This signal can be correlated to the viable cell density, because only viable cells can be polarized. Dead cells have a leaky membrane and cannot be polarized. This method is therefore insensitive to dead cells, cell debris, and microcarriers.
Accessories for Incyte Sensors
A measuring loops consist of an Incyte sensor and a pre-amplifier, which are factory calibrated. The Sensor Unit is connected to a Cell Density Controller and provides a variety of communication protocols, from analog 4-20 mA signal to digital OPC-XML-DA connection. Diverse accessories are available to complete the portfolio.
Cell Density Controllers and Accessories
Cell Density Cables
Cell Density Maintenance
Specific Applications
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App Note - Real-time cell density measurement for PAT applications
An application highlighting the use of Incyte and Dencytee in a PAT approach to monoclonal antibody (mAb) production. -
App Note - Online monitoring of CHO cell culture
A suite of Hamilton sensors is used to continuously monitor batch fermentation of CHO cells for tighter process control.
Resources
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Support Resource - Incyte Data Analysis Macro
Import offline data from the offline data worksheet and Incyte data into this macro for automatic generation of data analysis graphs. -
Support Resource - Incyte Protocol for Offline Measurement Correlation (Demo Protocol)
This guide details how to obtain the relevant offline measurements and online Incyte (permittivity) data of viable cell density to establish a correlation. -
Support Resource - Offline Data Worksheet for Incyte
Enter offline viable cell counts or measurements in the offline data worksheet for direct import into the data analysis makro.
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Will Incyte work with my cell line?
Incyte has been shown to work with cell lines ranging from cell culture to microbial fermentation. We have compiled a current list of known applications in our cell density applications ebook. Download a copy to learn more. -
How often do Incyte sensors need to be calibrated?
Hamilton recommends once per year with a validation at 6 months. -
How does Incyte measure viable cell density?
Incyte is a capacitance-based sensor responsive only to living cells.
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Incyte Cell Density Measurement Principles
Learn about how Hamilton Incyte cell density sensors can continuously measure viable cells. Incyte enables the dual frequency measurement mode, or a frequency scanning. -
Data Analysis Tools (Cell Density Pre-Amp versions)
Hamilton provides several tools to interpret cell density data and correlate it with offline measurements. Download them here. -
Incyte Sensors (Pre-amp Version) - Standard vs. Low Conductivity
There are several different options for Incyte cell density sensors based on liquid conductivity. Learn more about sensor selection / applications.
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