Cell Density Sensors

In-line viable cell and total cell density sensors

Close-up of a cell density sensor probe with white optical tip and cylindrical glass and metal body
Incyte Sensor in Bioreactor
incyte sensor kulmbacher
Stainless steel inline process sensor probe with threaded body, O-ring seal, and multi-pin connector

Monitor growth like never before with Hamilton in-line cell density sensors.

Gain a clear and present understanding into your bioprocess workflows with real time cell density sensors from Hamilton. Real-time cell density data helps to optimize critical biopharmaceutical production parameters and capture subtle changes in growth kinetics that are easily missed through time-consuming offline measurements.
Our Incyte Arc sensors directly measure viable cell density, while Dencytee sensors provide total cellular density based on transmission and reflection dual measurement principle. With unmatched precision, Hamilton’s cell density sensors support just about any cell type or cell culture environment.

  • The difference in measurement resolution between the off-line method and permittivity is like between a 60's TV and an 8K screen!

    We used the Incyte Arc Sensor to monitor the viable cell density. It is very easy to install in a bioreactor and connect to the monitoring software. After performing a baseline calibration in medium, you are ready to monitor your bioreactor run.

    Jort Altenburg, Ph.D
    Wageningen University, Netherlands
  • I could not be more pleased with my experience with Hamilton!

    My company has been using Hamilton probes for years and have always been happy with their performance! The team is always very helpful in trouble shooting issues and answering any questions that we may have about our probes. Overall, I could not be more pleased with my experience with Hamilton!

    Abigail Mann
    Bioprocessing Fermentation Associate at Heliae Development
  • We value the versatility and ease of integration of the Dencytee Arc system.

    In our QbD-centered PAT ecosystem, which requires frequent adaptation to different bioprocess modes and R&D setups, we especially value the versatility and ease of integration of the Dencytee Arc system.

    Prof. Dr. Lukas Neutsch
    Head Bioprocess Technology, Zurich University of Applied Sciences

Benefits of using Hamilton Cell Density sensors

  • Strengthen Process Control

    Real-time, in-line cell density measurements support rapid & actionable insights related to critical quality attributes.

  • Save Time and Reduce Contamination Risks

    In-line measurements eliminate the need for frequent cell culture sampling to measure cellular density off-line.

  • Support Enhanced Yields and Quality

    Schedule key cell culture process events like feeding and harvesting while precisely gauging cell growth and kinetics.

Explore All Cell Density Sensors

Select a cell density sensor type to see product recommendations from Hamilton.

Viable Cell Density Sensors

Incyte Arc

The Incyte Arc Expert sensor enables in-line, real-time viable cell density measurement, which is essential for achieving data-driven process optimization and control in bioprocessing

Total Cell Density Sensors

Dencytee Arc

Dencytee Arc sensors enable real-time, in-situ measurement of total cell density using transmittance and reflectance of near-infrared light in low and high cell density applications.

Single-Use Viable Cell Density Sensors

Incyte SU

The Hamilton’s Incyte SU sensor elements are pre-installed as integral part of the single-use container with a long shelf life.

How Does Cell Density Measurement Work?

In-line Total and Viable Cell Density measurements are now possible with a new breed of sensors. These cell density sensors measure cell growth in real-time without time-consuming manual and off-line steps. 

Technology Overview

Off line total and viable cell density Off-line Total and Viable Cell Density Viable cell density monitoring with Incyte Viable Cell Bio-Volume
with Incyte
Total cell density monitoring with Dencytee VCD Soft Sensor with Incyte Arc Total cell density monitoring with Dencytee Total Cell
Density with Dencytee
viability with incyte dencytee sensors Viability with Incyte and Dencytee
Benefits and Attributes

Discrete Data Points

Sampling Required

Contamination Risk

Enables Process Control

Real-Time Decisions

Continuous Measurement

Reduce Sampling

High

Low

Low

Low

Low

Additional Benefits
  • Cell Morphology Visualization
  • Growth Trend Interpolation
  • Detect Changes in Cell Size
  • Identify Apoptosis In-Situ
  • Online Calculation of Viable Cell Density that Correlates to Offline Measurement
  • Temperature Independent Total Cell Density Measurement
  • Online Viability Estimation
Measurement Output
  • Cell Count (cells mL-1)
  • Cell Viability (%)
  • Other Complex Measurements
  • Viable Cell Volume Expressed as Permittivity (pF/cm)
  • Viable Cell Count via Soft Sensor (Cells mL-1
  • Viable Cell Volume Expressed as Permittivity (pF/cm)
  • Total Cell Density Expressed as OD, AU, g L-1, Cells ml-1, NTU, arb.
    Unit, CFU, OD, PCV
  • Viability (%)
Requirements
  • Cell Counter and Chemicals
  • Off-line Samples
  • Operator
  • Sample Preparation
  • Incyte Arc Sensor
  • ArcAirTM
  • No Transmitter
  • Incyte Arc Sensor
  • ArcAirTM
  • ArcAir Data Modeling Software
  • Historical Process Data
  • Dencytee Arc Sensor
  • ArcAirTM
  • No Transmitter
  • Dencytee Arc Sensor
  • Incyte Arc Sensor
  • Integration at the PCS or SCADA

*Sampling required during initial modeling and model maintenance

Cell Density Sensors Resources

Browse app notes, user guides, specification documents, and more in our Knowledge Center.

Browse app notes, user guides, specification documents, and more in our Knowledge Center.

Browse app notes, user guides, specification documents, and more in our Knowledge Center.

Browse app notes, user guides, specification documents, and more in our Knowledge Center.

Browse app notes, user guides, specification documents, and more in our Knowledge Center.

Services

Installation, set-up, and calibration support directly on-site. Our field service team ensures your sensors and cables are installed and functioning correctly.

Support for the qualification of Hamilton equipment including complete documentation. Readying your process for GMP manufacturing activities.

Preventative maintenance and regular service contracts. Schedule on-site service dates to ensure your sensors are properly maintained and your process is optimized.

Diagnosing and resolution of process system problems on-site. Finding the root cause and presenting a solution to fit your needs.

User trainings and on-site trainings for technicians. Tailored training ranging from basic operation to advanced calibration and maintenance.

Backed by world-class service and support.

For over 75 years, the Hamilton name has been recognized globally for its unwavering commitment to quality. That pledge extends to creating long-term customer partnerships. We're proud to offer in-depth training courses, on-site field support, custom product builds, and so much more.

Let's Talk

Connect with a Hamilton expert to discuss solutions for your workflow. We are here to enable your success.

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