Innovative Technology for Nucleic Acid Size Selection

Hamilton, together with Coastal Genomics, developed the NIMBUS Select assay ready workstation with Ranger Technology for high-throughput nucleic acid size selection for use in NGS/MPS, cloning, gene synthesis, quality control, and other applications. The NIMBUS Select is the only system to fully automate agarose gel loading, electrophoretic analysis, and recovery of targeted DNA fragments for DNA size selection and analysis of nucleic acids. Up to 96 DNA samples may be processed in two hours, and up to 384 electropherograms may be created in a single run.

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Features and Benefits

Fully Automated 3-in-1 Tool

The NIMBUS Select is a versatile 3-in-1 tool, combining automated electrophoretic fragment analysis, size selection, and liquid handling in one compact footprint. This consolidated format improves efficiency and reduces active labor time while increasing throughput compared to manual methods. At the same time, Ranger Technology from Coastal Genomics eliminates closely-sized primers and adaptermers, and recovers tightly-defined DNA fragment lengths for high recovery yields.

Size Selection Mode

Up to 96 samples are automatically loaded into gels for electrophoresis, which is monitored and documented by a camera, and an electropherogram is generated. The target fragment range is then identified and extracted for downstream applications. Image-based monitoring and documentation is conducted in real-time, and this information is used to automatically control electrophoresis on a sample-specific basis. This process enables accurate, repeatable, and high-yield recoveries without user-based errors and variability, or off-target selection. Additionally, the high-throughput capacity simultaneously processes significantly more samples compared to manual methods in a hands-free format, so that users can refocus their attention on high-value activities.

  • Sample capacity: Up to 96 samples
  • Average run time: 2 hours
  • Functional range: 25 bp - 20 kbp

Analytics Mode

Up to 384 samples are automatically loaded into gels for electrophoresis, which is monitored and documented by a camera, and an electropherogram is generated. Analytics may be exported for use in LIMS-based systems, and human readable reports are also generated. This process enables accurate results and automates the sample handling associated with sample preparation. Additionally, the high-throughput capacity simultaneously processes significantly more samples compared to manual methods in a hands-free format, so that users can refocus their attention on high-value activities.

  • Sample capacity: Up to 384 samples
  • Average run time: 60 minutes
  • Functional range: 50 bp – 30 kbp

Liquid Handling

Air displacement pipetting using Hamilton tips with CO-RE (Compressed O-Ring Expansion) technology provides superior measurement accuracy, precision, and reproducibility without tip distortion or aerosol generation during tip pick-up and ejection.

Hamilton automation is evolving NGS workflows

Read “Automated Size Selection for Short Cell-Free DNA Fragments Enriches for Circulating Tumor DNA and Improves Error Correction During Next Generation Sequencing” written by Dr. Hunter Underhill, who used the NIMBUS Select to perform much of the research discussed in the article.

Dr. Underhill will be giving a presentation concerning his recent research at SNO18 in New Orleans, which will be held from November 15 - November 18, 2018.

Read the Article

Specifications

All specifications are based on the Microlab NIMBUS96.

Dimensions
Length41.0 in (1041 mm)
Width28.0 in (711 mm)
Height34.5 in (876 mm)

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