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AutoLys STAR

AutoLys

Automated Lysis & DNA Extraction: Eliminate your sample lysis bottleneck.

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AutoLys STAR

Hamilton’s Microlab AutoLys STAR liquid handling workstation provides a fully automated solution for lysis and DNA extraction from dried blood and saliva stains, swabs, paper punches, cigarette butts, hard tissues and other forensic specimens residing on solid surfaces.

Major bottlenecks in the DNA isolation process are sample preparation and lysis steps.
These early steps are not only time consuming but possible sources of human error and cross contamination.

The AutoLys workstation developed by Hamilton provides a unique solution that fully automates the entire sample lysis and lysate recovery process. Additionally, the Autolys workstation can be configured to automate the complete process from raw sample lysis through DNA purification.

Revolutionary AutoLys channels and Autolys tubes have been developed to fully automate
the lysis process while guaranteeing sample integrity, traceability and DNA yields.
The unique AutoLys tube carries out hands-free, uninterrupted preparation of cleared lysates separated from solid material.

The AutoLys is designed to prepare lysates from a wide diversity of samples for clinical, forensic, veterinary and food testing applications.
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BENFITS

  • Labor Savings: eliminates tedious spin column handling and manual pipetting steps.
  • Time Savings: the AutoLys STAR will perform the Lysis process completely unattended.
  • Enhanced Sample Integrity: minimizes risk of inadvertent contamination; proprietary AutoLys Tube with 2-D barcode ensures chain-of-custody is maintained.
  • High DNA yields:  Maximize DNA recoveries while maintaining full chain of custody

The AutoLys tubes combined with the AutoLys STAR system enables full sample traceability. After a sample or an exhibit is processed and placed in AutoLys tubes, lysis is performed fully automated by the AutoLys STAR. Subsequent DNA purification can be performed on the same deck or on a different platform.

Processing workflow using the AutoLys tubes and AutoLys STAR. Traces are directly linked to a tube by matching barcodes, enabling full traceability during extraction. The integrated filter allows automated separation of lysate and trace carrier. The complete workflow takes place in a single vial.

workflow autoLys

autoLys-accessories-1The AutoLys heater shaker has been designed to tightly fit the AutoLys tubes for optimal heat transfer and shaking efficiency.
Temperature: Ambient to 105°C. Speed: 0-1800 rpm. Amplitude: 3 mm.
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autoLys-accessories-2easyCode 2-D barcode reader is ultra-fast, high-resolution 2-D barcode reader.
Provides total traceability throughout the process.
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autoLys-accessories-3Integrated, dual SBS plate positions, on-deck centrifuge with swing-out rotor. AutoLys tube racks are centrifuged at 2000 xG.
Allows maximum lysate recovery for maximum DNA yields.
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accessories-5

AutoLys Tubes: Hamilton’s AutoLys Tube is a novel, automation-friendly consumable designed explicitly for forensic DNA sample lysis and extraction on the AutoLys STAR. The proprietary tube is DNAase-free, DNA Free and consists of a 3-part assembly:

  • Outer tube with 2-D barcode
  • Inner tube with flow-thru filter
  • Threaded cap
  • Process and Filter up to 600 μL of Lysate

 

 AutoLys workstations are available in several base configurations to be adapted to user specific workflows.

Pipetting
Channels

AutoLys
Channels

Autoload

Centrifuge

2-D Barcode
Reader

Deck
Capacity*

AutoLys STAR 4+4

4

yes

yes

yes

39 tracks
(15 tracks used by centrifuge)

AutoLys STAR 8+4

8

4

yes

yes

yes

39 tracks
(15 tracks used by centrifuge)
 

AutoLys STARplus 8+4

8

4

yes

yes

yes

55 tracks
(15 tracks used by centrifuge)

* As example: Carriers with Hamilton shakers occupy 7 tracks, Carriers for SBS format labware or AutoLys racks occupy 6 Tracks and Tube or Reagent trough carriers occupy 1 Track each. The configuration of each system is flexible and is adapted to your workflow or protocol. A possible configuration to lyse up to 96 samples consists of: 1 Tip Carrier (up to 480 tips), 3x Sample Tube carriers for up to 96 tubes, 1 Reagent Carrier with up to 5 x 60 mL troughs, 1 Shaker Carrier with 4x AutoLys Shakers, 1 Shaker Carrier with 1x AutoLys Shaker and 2 Positions for AutoLys Racks, 1 AutoLys Rack carrier with 4 positions and 1 easyCode Carrier.