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Tip Based Extraction

Extraction_Tip_Based_1

The purification of genomic DNA is the imperative first step to genetic-based tests including pharmacogenomics, prenatal and newborn screening, genotyping, diagnostic studies and forensics.

Though there are numerous clinical sample types from which to isolate genomic DNA, whole blood remains a common and more consistent source of genomic material compared to saliva and swab samples and is used by many research and clinical laboratories. Whole blood offers the consistently high yields over wide populations with high quality regardless of the preservative method.

Akonni Biosystems has developed an automated, high throughput TruTip extraction technology as a simple and affordable solution for fast and efficient isolation of human genomic DNA from fresh and frozen blood.

The purification of genomic DNA is the imperative first step to genetic-based tests including pharmacogenomics, prenatal and newborn screening, genotyping, diagnostic studies and forensics. Though there are numerous clinical sample types from which to isolate genomic DNA, whole blood remains a common and more consistent source of genomic material compared to saliva and swab samples and is used by many research and clinical laboratories. Whole blood offers the consistently high yields over wide populations with high quality regardless of the preservative method. Akonni Biosystems has developed an automated, high throughput TruTip extraction technology as a simple and affordable solution for fast and efficient isolation of human genomic DNA from fresh and frozen blood.

The TruTip technology uses a porous binding matrix embedded in a pipette tip (Figure 1) with chaotropic salt chemistry and eliminates the need for costly vacuum filtration, centrifugation, or magnetic rod systems. The purified sample is free of inhibitors and contaminants and ready for any downstream detection method. Automation of the extraction process on the Hamilton STAR platform offers a dependable, cost-effective solution for high throughput workloads. The entire process is performed on-board the instrument including a proteinase K incubation using the Hamilton HHS2 heater/shaker which allows the user to load sample tubes onto the platform and retrieve the final extracted samples at the completion of the run. The 96-channel arm allows for high throughput processing of 96 samples in less than 60 minutes, for a total of 768 samples per 8-hour shift. Herein we demonstrate the high precision of the automated extraction system in processing genomic DNA from low volume whole blood samples and its advantage over competitors.

Features and Benefits

  • Fast extractions – 96 in less than 60 minutes, for a total of 768 samples per 8-hour shift
  • High quality extraction products with high reproducibility
  • Simple deck layout with throughput flexibility on the STARlet, STAR and STARplus platforms
  • Eliminates the need for costly vacuum filtration, centrifugation, or magnetic rod systems
  • Fully automated protocol performed on-board the instrument allows user to set-up and walk away