Automation of high-throughput PCR preparation for high-resolution HLA typing by Next-Generation Sequencing using MagPip on the Hamilton VANTAGE

App Note Mag Pip HLA Typing Cover

Streamlined HLA Genotyping with High-Precision Automation on the Hamilton® VANTAGE

High-resolution HLA typing is critical in donor matching and immune-related diagnostics. To meet increasing throughput and quality demands, MVZ Martinsried implemented an automated PCR setup using MagPip low-volume pipetting on the Hamilton® VANTAGE Liquid Handling Platform. The result: reliable sub-microliter pipetting, streamlined operations, and significantly improved reproducibility across 384-well PCR workflows.

Key Takeaways

  • Precision at scale: MagPip delivers 0.35 µL accuracy and consistency, ideal for high-throughput multiplex PCR applications.
  • Integrated automation: Primer mix preparation, dispensing, and PCR setup are unified on one system, reducing manual steps and minimizing cross-device variability.
  • Improved sequencing readiness: Sample reanalysis rates were reduced by over 60%, enabling more efficient NGS runs with fewer dropouts.

Read the Full Application Note to Learn More.