The Power of In-Line Cell Density Measurement

On-line, or in-situ, measurement is the pinnacle of process monitoring and necessary for true process control as evidenced with control of pH and dissolved oxygen, common for most biological processes. In fact, many parameters can be monitored but those directly related to cell physiology are typically time consuming off-line measurements that provide only a reactionary window into the past. Incyte Arc and Dencytee sensors provide a means for directly measuring viable cell density and total cell density in real-time, meeting the increasing need for PAT in the biopharmaceutical industry.

viable cell density sensor measurement principle

Viable Cell Density Sensors

Incyte Arc sensors provide a means for directly measuring viable cell density in real-time, meeting the increasing need for PAT in the biopharmaceutical industry. Clear, instantly available information ensures critical events that could have been missed between off-line samples are now immediately recognizable.
Dencytee sensor

Total Cell Density Sensors

Total cell density measurement by the Dencytee sensor is based on optical density, commonly known as the turbidity of a suspension at near-infrared (NIR) wavelengths. All particles and molecules that scatter the NIR light will be detected. This optical density signal can be correlated to the total cell density measured by different offline measurement techniques.

How Does Cell Density Measurement Work?

Many parameters like pH and dissolved oxygen can be monitored in-line but those directly related to cell physiology are typically time consuming offline measurements that require many manual steps. Total and Viable Cell Density measurement is now possible in-line with a new breed of sensors. Learn more about saving time and effort and increasing productivity and process understanding with in-line cell density sensors in the video below.

Will Hamilton Cell Density Sensors Work With My Process?

Hamilton cell density sensors are compatible with many types of cell lines and microbial strains, process types, and industries. Read more below or download our Cell Density Applications eBook to see real data with different cell lines and process types.

Technology Overview

Off line total and viable cell density Off-line Total and Viable Cell Density Viable cell density monitoring with Incyte Viable Cell Bio-Volume
with Incyte
Total cell density monitoring with Dencytee VCD Soft Sensor with Incyte Arc Total cell density monitoring with Dencytee Total Cell
Density with Dencytee
viability with incyte dencytee sensors Viability with Incyte and Dencytee
Benefits and Attributes

Discrete Data Points

Sampling Required

Contamination Risk

Enables Process Control

Real-Time Decisions

Continuous Measurement

Reduce Sampling

High

Low

Low

Low

Low

Additional Benefits
  • Cell Morphology Visualization
  • Growth Trend Interpolation
  • Detect Changes in Cell Size
  • Identify Apoptosis In-Situ
  • Online Calculation of Viable Cell Density that Correlates to Offline Measurement
  • Temperature Independent Total Cell Density Measurement
  • Online Viability Estimation
Measurement Output
  • Cell Count (cells mL-1)
  • Cell Viability (%)
  • Other Complex Measurements
  • Viable Cell Volume Expressed as Permittivity (pF/cm)
  • Viable Cell Count via Soft Sensor (Cells mL-1
  • Viable Cell Volume Expressed as Permittivity (pF/cm)
  • Total Cell Density Expressed as OD, AU, g L-1, Cells ml-1, NTU, arb.
    Unit, CFU, OD, PCV
  • Viability (%)
Requirements
  • Cell Counter and Chemicals
  • Off-line Samples
  • Operator
  • Sample Preparation
  • Incyte Arc Sensor
  • ArcAirTM
  • No Transmitter
  • Incyte Arc Sensor
  • ArcAirTM
  • ArcAir Data Modeling Software
  • Historical Process Data
  • Dencytee Arc Sensor
  • ArcAirTM
  • No Transmitter
  • Dencytee Arc Sensor
  • Incyte Arc Sensor
  • Integration at the PCS or SCADA

*Sampling required during initial modeling and model maintenance

Resources

Hamilton's knowledge base provides a range of explanatory articles, frequently asked questions, and document downloads.
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