The Power of On-Line Measurement

The value of continuous, on-line measurement has been long known in the bioprocessing world as evidenced by the prominence of on-line pH, DO, and similar sensors. Understanding cell growth is possibly the most important parameter of a bioprocess (cell culture or fermentation) and yet is commonly measured at discrete time points only. With Hamilton's selection of cell density sensors, the power of on-line measurement can be utilized for continuous, real-time viable and total cell density analysis.

Incyte

Incyte measures permittivity of viable cells. The signal can be correlated to the viable cell density (VCD) measured offline. This on-line VCD sensor can be used directly in the bioreactor for real-time process control, eliminating the need for offline sampling.

Dencytee

Total cell density (TCD) measurement by the Dencytee sensor is based on the optical density, or the turbidity of a suspension at NIR wavelengths. The Dencytee sensor has a wide measuring range of TCD.

On-line Cell Density Measurement

Many parameters like pH and dissolved oxygen can be monitored online but those directly related to cell physiology are typically time consuming offline measurements that require many manual steps. Total and Viable Cell Density can now be measured on-line with a new breed of sensors. Learn more about saving time and effort and increasing productivity and process understanding with on-line sensors in the video below.

Off-line Measurements

Typical analysis of viable cell density or cell viability is done through offline sampling and manual or automated analysis. Off-line analysis methods have many deficiencies that lead to large amounts of time and effort and considerable risk and error.

Will Hamilton Cell Density sensors work with my process?

Hamilton Cell Density sensors are compatible with many types of cell lines and microbial strains, process types, and industries. Read more below or download our Cell Density Applications eBook to see real data with different cell lines and process types.

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Compatible Organisms

  • Mammalian
  • Yeast
  • Filamentous fungi
  • Bacterial

Compatible Process Types

Incyte and Dencytee are compatible with all typical bioprocessing types.

  • Batch
  • Fed-batch
  • Continuous/Perfusion

Industries

  • Biopharma
  • Brewing

Arc View Controllers

Hamilton’s specialized Arc View Controllers allow you to visualize and record data from and communicate with Incyte and Dencytee sensors from a single tool. Viable Cell Density (Incyte) and Total Cell Density (Dencytee) data can be viewed on one graph for real-time visualization of cell viability. Hamilton offers several different controller options depending on your needs. See all Cell Density Controllers.

Specific Applications

Read the latest application notes to learn how scientists are automating their workflows.

Resources

Hamilton's knowledge base provides a range of explanatory articles, frequently asked questions, and document downloads.
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