HC-75 HPLC Columns

HC-40 and HC-75 resins are soft gel column packings designed for oligosaccharide separations. The mobile phase of these columns is water without salts or buffer. This simplifies mobile phase preparation, reduces cost, and broadens the use of available detectors. Each of these columns has distinct retention characteristics and is designed for specific separations.

Application Examples: Oligosaccharides (degree of polymerization ≤ 8)

HC-75 Columns
HC-75 Guard Columns
HC-75 Bulk Resin

HC-75 Overview

HC-75 resins are soft gel column packings designed for oligosaccharide separations. The mobile phase of these columns is water without salts or buffer. This simplifies mobile phase preparation, reduces cost, and broadens the use of available detectors. Each of these columns has distinct retention characteristics and is designed for specific separations.

How to Choose Column Specifications for Your Application

Choosing Column Specifications

The sample amount needed to be purified or separated for analysis will dictate which scale of chromatography is needed. In general, the scales of chromatography are microbore, analytical, semi-preparative, and preparative. Nanoscale and microscale are smaller scale ranges but are not covered in Hamilton’s product offerings. READ MORE

Tech Center

HPLC Column Care & Troubleshooting

HPLC Resin Specifications

HPLC Guard Cartridge Holder Assembly - Stainless Steel

Safety Data Sheets

Specific Applications

Bio Fermented Alcohol Detection Using Isocratic Cation Exchange HPLC (HC-75 H+)

Concern over detrimental greenhouse gases produced from the combustion of fossil fuels has been growing for the past few decades.
Raffinose Family of Oligosaccharides by Cation Exchange HPLC (HC-75 Ca2+)

A ligand exchange mechanism is explored as a technique to resolve Verbascose, Stachyose, Raffinose, and Melibiose.
Saccharide Identification in Honey by Cation Exchange HPLC (HC-75 Pb2+)

Is the honey you consume a prime target for adulteration with refined sugars? Read our app note on saccharide identification

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Resources

Hamilton's knowledge base provides a range of explanatory articles, frequently asked questions, and document downloads.

Reference Guide - HPLC Columns

Learn how to choose a column, how to maximize the life of a column through proper care, cleaning, and restoration.
SDS - HC-75 (resin, dry)

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