HC-75

cation exchange column

HC-75 resins are soft gel column packings designed for oligosaccharide separations. The mobile phase of these columns is water without salts or buffer. This simplifies mobile phase preparation, reduces cost, and broadens the use of available detectors. Each of these columns has distinct retention characteristics and is designed for specific separations.


 

Application Examples: Oligosaccharides (degree of polymerization ≤ 8)

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HC-75 Columns

Cation exchange columns with ligand exchange as the primary mechanism of separation.
Prp 1

HC-75 Guard Columns

Protect your HC-75 column.
Guard columns

HC-75 Overview

HC-75 resins are soft gel column packings designed for oligosaccharide separations. The mobile phase of these columns is water without salts or buffer. This simplifies mobile phase preparation, reduces cost, and broadens the use of available detectors. Each of these columns has distinct retention characteristics and is designed for specific separations.

How to Choose Column Specifications for Your Application

Tech Center

HPLC Column Care & Troubleshooting
HPLC Resin Specifications
HPLC Guard Cartridge Holder Assembly - Stainless Steel
Safety Data Sheets

HC-75 Resources

Browse app notes, user guides, specification documents, and more in our Knowledge Center.

Browse app notes, user guides, specification documents, and more in our Knowledge Center.

HAM Reference Guide HPLC Columns

Guide

Reference Guide - HPLC Columns

Learn how to choose a column, how to maximize the life of a column through proper care, cleaning, and restoration.
HAM SDS HC 75 resin dry

SDS

SDS - HC-75 (resin, dry)

Download the Safety Data Sheet for Hamilton HC-75 (Dry), used as a filter media in HPLC applications. Includes key safety, handling, and first aid information.
High Quality Wines

Application Note

Precision You Can Taste: Simplifying Organic Acid Analysis in Wine (HC-75)

The HC-75 H+ Form chromatography resin is specifically engineered for the analysis of six critical fermentation analytes the organic acids (tartaric, malic, lactic, and citric acids) while accurately assessing the key carbohydrates (glucose and glycerol).

Browse app notes, user guides, specification documents, and more in our Knowledge Center.

cation exchange column

Article

USP "L" Column Listings

Explore Hamilton’s qualifying USP “L” columns.

Browse app notes, user guides, specification documents, and more in our Knowledge Center.

HPLC FAQ

FAQ

How Do I Connect a Stainless Steel Guard Column to My HPLC Column?

Connecting a guard column to an HPLC column is straightforward, but requires different steps based on the HPLC instrument and its configuration. Click the headline above to read this step-by-step guide.
HPLC FAQ

FAQ

How Do I Connect a PEEK Guard Cartridge to my HPLC Column?

The process of connecting a guard cartridge to a HPLC column is straightforward, but requires different steps based on the HPLC instrument and its configuration. Click the headline above to read this step-by-step guide.
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