Separation of Five Steroids (PRP-C18)
Steroids represent a chemically distinct class of hormones with wide-ranging biological functions. Synthetic derivatives of endogenous steroid prototypes are used medically in birth control and in the treatment of asthma, arthritis, inflammation and osteoporosis.
Steroids share a characteristic, polycyclic structure and have varying degrees of lipophilicity (log P). In this study, a reversed phase HPLC method was developed for separation of five steroid hormones with partition coefficients ranging from 1.47 (cortisone) to 4.5 (pregnenolone) on a Hamilton PRP-C18 HPLC column.
