PRP-X300 7 µm, 4.1 x 150 mm, PN: 79464

Ion Exclusion, Stainless steel

Part # 79464

PRP-X300 (PN: 79464) with a sulfonated poly (styrenedivinylbenzene) support separates samples via a mixed-mode mechanism. Separation on the PRP-X300 is accomplished by three modes: hydrogen bonding, reversed-phase, and ion exclusion.

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PRP-X300 7 µm, 4.1 x 150 mm, PN: 79464 Details

Hamilton PRP-X300 columns offer an easy, rapid way to separate closely related alcohols and organic acids. The sulfonated poly(styrenedivinylbenzene) support separates samples via a mixed-mode mechanism. Separation on the PRP-X300 is accomplished by three modes: hydrogen bonding, reversed-phase, and ion exclusion. PRP-X300 column selectivity can be altered by changing the pH of the buffer or adding an organic modifier (e.g., methanol, acetonitrile). The support’s stability to organic solvents makes it possible to analyze samples that are too highly retained on conventional ion exclusion supports.

The high-performance packing ensures narrow peaks under isocratic conditions. The use of isocratic conditions allows samples to be analyzed one after another without waiting for column re-equilibration. Most samples require only minimal preparation before injection, increasing sample throughput in the lab.
 

Application Examples: Organic acids and alcohols

Scientific Citations

PRP-X300 7 µm, 4.1 x 150 mm, PN: 79464 Specifications

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Separation Mode Ion Exclusion
Stationary Phase PRP-X300
Format HPLC Column
Hardware Inner Diameter 4.1 mm
Hardware Length 150 mm
Particle Size 7 µm
USP Classification USP L22
Packing Material Type PSDVB/Sulfonic Acid
Pore Size 100 Å
Particle Surface Area (m2/g) 360
Pore Volume (cm3/g) 0.748
Hardware Material Stainless Steel
Max Pressure 5,000 psi
Exchange Capacity 0.17 meq/gm
Mobile Phase Limits pH 1-13, 0-100% aqueous, organic modifier
Buffer Strength 0.0-0.5N
Temp Limits 5-60°C
Restoration Inject several times with 100 µL of 1 N nitric acid
Compound Classification Organic Acids/Alcohols
UOM 1 EACH

PRP-X300 7 µm, 4.1 x 150 mm, PN: 79464 Resources

Browse app notes, user guides, specification documents, and more in our Knowledge Center.

HAM SDS PRP X300 resin dry

SDS

SDS - PRP-X300 (resin, dry)

Download the SDS for Hamilton PRP – X300 Resin (Dry), an HPLC filter media. Review important safety information including handling guidelines, health hazards, and first aid procedures.
Separation of Halo Acetic Acids

Application Note

Separation of Halo Acetic Acids by Ion Exclusion (PRP-X300)

In this app note. we explore a method that isolates fluoro, chloro, bromo, and iodoacetic acids from water samples which highlights the effectiveness and robustness of the Hamilton PRP-X300.
cation exchange column

Article

USP "L" Column Listings

Explore Hamilton’s qualifying USP “L” columns.

Browse app notes, user guides, specification documents, and more in our Knowledge Center.

HAM SDS PRP X300 resin dry

SDS

SDS - PRP-X300 (resin, dry)

Download the SDS for Hamilton PRP – X300 Resin (Dry), an HPLC filter media. Review important safety information including handling guidelines, health hazards, and first aid procedures.
Separation of Halo Acetic Acids

Application Note

Separation of Halo Acetic Acids by Ion Exclusion (PRP-X300)

In this app note. we explore a method that isolates fluoro, chloro, bromo, and iodoacetic acids from water samples which highlights the effectiveness and robustness of the Hamilton PRP-X300.

Browse app notes, user guides, specification documents, and more in our Knowledge Center.

cation exchange column

Article

USP "L" Column Listings

Explore Hamilton’s qualifying USP “L” columns.
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