Nucleotides on 150 x 4.1 mm PRP-X100, P/N 79434
Application #74
HPLC Column
| Packing Material | PRP-X100 |
|---|---|
| Part Number | PRP-X100 10 µm, 4.1 x 150 mm, PN: 79434 |
Chromatographic Conditions
| Injection Volume | 20 µL |
|---|---|
| Detection | UV at 254 nm |
| Column Dimensions | 150 x 4.1 mm |
| Eluent A | 25 mM Citric Acid |
| Eluent A pH | 5.4 |
| Eluent B | 17:3 25 mM Citric Acid : Acetonitrile |
| Eluent B pH | 5.4 |
| Flow Rate | 2.0 mL/min |
Compounds
| 1 | Cytidine 5'-Monophosphate (CMP) |
|---|---|
| 2 | Uridine 5'-Monophosphate (UMP) |
| 3 | Guanosine 5'-monophosphate (GMP) |
| 4 | Thymidine 5'-monophosphate (TMP) |
| 5 | Adenosine 5'-monophosphate (AMP) |
