viable cell density sensor measurement principle

Incyte Viable Cell Density Sensors - More Data, More Control, More Possibilities

Continuous in-line, or in-situ, data on viable cell density allows for real-time process control. With off-line data, decisions can only be made at the time of sample analysis, resulting in imprecise timing or missing events entirely.

Permittivity is quickly becoming the industry standard for the measurement of viable cell volume because of its robust real-time data. It correlates well to off-line cell counting in the crucial exponential phase without the risk of counting errors. When paired with ArcAir Data Modeling software, this correlation can also be made throughout the process. Incyte Arc sensors can be used to increase process quality across many cell lines, process types, and scales.

Incyte SU - single use conductivity sensor

Incyte SU (Single-Use)

Hamilton's game-changing viable cell density sensors are now available in a patch-based format that is welded directly into a bioreactor bag.
Incyte Arc Cell Density sensor

Incyte Arc

Incyte Arc sensors enable real-time, in-situ measurement of viable cells, making it possible to detect events and respond in real time without increased off-line sampling.

Incyte Viable Cell Density Sensors In Action

From increasing yield to shortening process time, the real power of permittivity is in your hands. As shown in the examples below, ask yourself what you could do with continuous process data.

Process understanding

Process Understanding

Expand your PAT capabilities with permittivity and gain process insights such as cell physiology changes through the use of multi-frequency scan.

Automated process control

Automated Control

Precisely time key process events such as feeding, induction, and harvest for lower production cost, increased yield, and enhanced product quality.

Consistent quality

Consistent Quality

Compare permittivity data to ensure batch-to-batch and scale-up reproducibility.

Continuous process improvement

Continuous Process Improvement

Gather on-line viable cell density data every run to enable continuous optimization.

Process optimization real time

Process Optimization

Real-time data means never missing a key process event. Use this data to develop enhanced process decisions for increased efficiency and prolonged productivity. Alternatively, use ArcAir Data Modeling software for real-time understanding of legacy off-line counts.

viable cell density soft sensor

Viable Cell Density Soft Sensor

AADM calculates real time viable cell density that correlates to offline measurement and closes all gaps between the measuring points.

The Power of Permittivity for VCD

Permittivity measurements are the most reliable method of monitoring viable cell density (VCD). This measurement is immediately affected by changes in viable cell density and can be used to time process-specific actions for maximum yield. Permittivity can also be used to detect changes in cell physiology and is the most immediate method for determining the beginning of the cell death phase.

viable cell density permittivity

Continuous Data

Incyte Arc sensors provide a real-time measurement of viable cell density with continuous permittivity data. Off-line samples only provide limited, error-prone data points, so process events may be missed. Real-time permittivity reveals changes in cell physiology, so advanced process control can be automatically triggered for process optimization.

Available Tools for VCD Sensors

Off line total and viable cell density Off-line Total
and Viable
Cell Density
Viable cell volume with incyte Viable Cell
with Incyte
Vcd soft sensor with incyte arc VCD Soft
Sensor with
Incyte Arc
Benefits and Attributes

Discrete Data Points

Sampling Required

Contamination Risk

Enables Process Control

Real-Time Decisions

Continuous Measurement

Reduce Sampling




Additional Benefits
  • Cell Morphology Visualization
  • Growth Trend Interpolation
  • Detect Changes in Cell Size
  • Identify Apoptosis In-situ
  • Online Calculation of Viable Cell Density that Correlates to Offline Measurement
Measurement Output
  • Cell Count (cells mL-1)
  • Cell Viability (%)
  • Other Complex Measurements
  • Viable Cell Volume Expressed as Permittivity (pF/cm)
  • Cell Count (cells mL-1)
  • Cell Viability (%)
  • Viable Cell Volume Expressed as Permittivity (pF/cm)
  • Cell Counter and Chemicals
  • Off-line Samples
  • Operator
  • Sample Preparation
  • Incyte Arc Sensor
  • ArcAirTM
  • No Transmitter
  • Incyte Arc Sensor
  • ArcAirTM
  • ArcAir Data Modeling Software
  • Historical Process Data

*Sampling required during initial modeling and model maintenance

Will Hamilton Cell Density Sensors Work with My Process?

Cell Density applications ebook

Review Hamilton's Cell Density Applications Ebook

Hamilton cell density sensors are compatible with many types of cell lines and microbial strains, process types, and industries. Read more below or download our Cell Density Applications eBook to see real data with different cell lines and process types.

Start a Conversation

After starting a conversation with a Hamilton's team member, you will have access to an applications specialist to discuss the potential of Hamilton Incyte Arc sensors in your bioprocess.

Leverage Hamilton Expertise

Utilize Hamilton’s Application Team and proven evaluation process for rapid implementation and robust product evaluation.

Sensors for Single Use environments

Sensors for Single-Use

Hamilton has adapted its trusted sensor technology to single-use environments, including solutions for pH, dissolved oxygen, conductivity, and viable cell density. Now available for bag manufacturers and other integrators.

View Incyte Single-Use Sensors


Specific Applications


Hamilton's knowledge base provides a range of explanatory articles, frequently asked questions, and document downloads.
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