Cell Density Measurement Guidelines
Process Analytical Technology (PAT) is crucial in any biopharma application. One Key Process Indicator critical to PAT implementation is how many cells are present (also known as cell density). Monitoring cell density is the first step toward in-situ control at the bioreactor. Cell density can be measured two ways: using only the living or viable cells or using the total cells, including dead cells.
| Sensor Type | In-line viable biomass | In-line total biomass |
| Signal | Capacitance | Optical density |
| Correlation | Only viable cells using a cell factor | Total cells (and cellular debris/particles) using an in-process model builder |
| Application Considerations | No influence by media composition or microcarriers. High resolution for a wide range of cell concentrations. In-depth data analysis can show changes in cell physiology. Minimum concentration thresholds apply. | Combination of transmission and reflection measurement modes enables high precision in a wide range of biomass applications. Wide measurement range compared to sensors only using one measurement mode. Maximum concentration thresholds apply. |
| Robust Measurement | Adherent and suspension-based processes | Suspension-based processes |
| Ideal Systems | Mammalian, insect, bacterial/yeast, fungal | Bacteria and yeast; use in conjunction with Incyte in other systems |
| Applications | Learn more through the Incyte Arc application notes | Learn more through the Dencytee Arc application notes |
Related Content:
Learn more about the measurement principles behind Dencytee Arc and Incyte Arc.
Find more cell density applications in the Cell Density eBook.
Check out the Cell Density Sensor brochure.
Explore other in-line sensors!