Split/splitless injection consists of a heated chamber with a glass liner into which the sample is injected through the septum. A microsyringe is used to inject the sample through a rubber septum into a flash vaporizer chamber at the head of the column. The sample vaporizes to form a mixture of carrier gas, vaporized solvent and vaporized analytes. In the split mode, only a proportion of this mixture reaches the column but most exits through the split outlet. This avoids overloading the column. In the splitless mode, the split vent is closed so that the vaporized analyte passes onto the column. This mode is more sensitive and adequate for trace analysis.
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