PRP-C18
PRP-C18 columns have genuine pH and chemical stability. The stationary phase stands up to prolonged exposure to concentrations as high as 1 M NaOH and 1 M H2SO4, with no measurable decrease in performance. The support does not strip, bleed, or dissolve at any pH. It can therefore perform reliably and reproducibly throughout the extended life of the column, regardless of mobile phase conditions. Unlike a silica C18 column, the PRP-C18 is resistant to temperatures above 100°C.
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PRP-C18 Columns
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PRP-C18 Guard Columns
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PRP-C18 Bulk Resin
PRP-C18 Overview
High efficiency separations at any pH
The Hamilton PRP-C18 is a new HPLC column designed to provide high-efficiency, reversed-phase separations over an extended column life in nearly any mobile phase or pH. The rigid stationary phase has excellent mechanical and thermal stability (> 100°C), does not shrink or swell, and is completely inert to most conditions commonly encountered in reversed-phase chromatography. The PRP-C18 is a durable and effective column for general purpose HPLC and especially well-suited for specialized applications in the clinical, pharmaceutical, environmental, food, forensics, and life sciences industries. For additional information, please refer to the PRP-C18 Product Datasheet.
How to Choose Column Specifications for Your Application
Tech Center
Specific Applications
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Oligonucleotide Purification by Reversed-Phase Ion Pairing Chromatography (PRP-C18)
A comprehensive investigation of purifying oligonucleotides. -
Separation of Fat Soluble Vitamin K Congeners in Under 6 Minutes (PRP-C18)
With the growing utility of the K vitamin series of molecules, a need appears relevant for both food labeling of constituents and for research based separation of the natural and synthetic compounds of vitamin K. -
Separation of Selected Components of Ylang-Ylang Oil (PRP-C18)
In this study of an essential oil, we have developed an HPLC method for the separation of 6 well-known markers for the determination of purity of Ylang-Ylang oil. -
Reversed-Phase Separation of Isoflavones and Mycotoxins in Soy Beans (PRP-C18)
Soy beans can contain a fungal metabolite and mycotoxin, Zearalenone (ZEA), which cause an increase in the proliferation of certain tumor cells
Resources
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Reference Guide - HPLC Columns
Learn how to choose a column, how to maximize the life of a column through proper care, cleaning, and restoration. -
PRP-C18 Product Datasheet
Explore the benefits of the PRP-C18 HPLC Column.
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How do I restore my HPLC column?
Performance will inevitably degrade as the column is used, however this procedure may restore the performance of a column that has become fouled. Learn more in this step-by-step guide. -
How Do I Connect a Stainless Steel Guard Cartridge to My HPLC Column?
The process of connecting a guard cartridge to an HPLC column is fairly simple, but requires different steps based on the HPLC instrument and the guard cartridge configuration. Click to read our step-by-step guide. -
How Do I Install My HPLC Column?
Connecting an chromatography column is not difficult, but if the proper steps aren't followed, chromatographic results can be compromised. Follow this step-by-step guide to get it done right.
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USP "L" Column Listings
Explore Hamilton’s qualifying USP “L” columns. -
HPLC Guard Columns, HPLC Guard Cartridges
HPLC guard columns and guard cartridges protect analytical, semi-prep and preparative HPLC columns. They help by removing particulate contaminants and highly absorptive compounds from samples, prolonging column life.
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