HC-75 (H+) 9 µm, 7.8 x 100 mm, PN: 79547

Cation Exchange, Stainless steel

Part # 79547

HC-75 is a poly (styrene-divinylbenzene) copolymer with 7.5% cross-linkage.  HC-75 is a soft gel column designed for oligosaccharide separations. The HC-75 column (PN: 79547) in hydrogen (H+) form is excellent in analyzing carbohydrates in combination with organic acids, alcohols, fatty acids, and amino sugars.  

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HC-75 (H+) 9 µm, 7.8 x 100 mm, PN: 79547 Details

HC-75 is a poly (styrene-divinylbenzene) copolymer with 7.5% cross-linkage.  The HC-75 column in hydrogen (H+) form is excellent in the separation of sugars in combination with organic acids, alcohols, and glycerol, which is essential in QC and monitoring tests for fuel ethanol fermentation analysis.  The mobile phase is only water with an acid modifier if needed.  This reduces cost and broadens the use of available detectors.

Application Examples: Oligosaccharides (degree of polymerization ≤ 8)

Scientific Citations

HC-75 (H+) 9 µm, 7.8 x 100 mm, PN: 79547 Specifications

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Separation Mode Cation Exchange
Stationary Phase HC-75 (H+)
Format HPLC Column
Hardware Inner Diameter 7.8 mm
Hardware Length 100 mm
Particle Size 9 µm
USP Classification USP L17
Packing Material Type PSDVB/Sulfonic Acid
Pore Size Gel-Type
Hardware Material Stainless Steel
Max Pressure 400 psi
Exchange Capacity 5 meq/gm
Mobile Phase Limits 100% Water 0-40% Acetonitrile
Buffer Strength 0.0-0.5N
Temp Limits 25-90°C
Restoration Flush with 0.1 N sulfuric acid at 0.1 mL/min overnight.
Compound Classification Carbohydrates
UOM 1 EACH

HC-75 (H+) 9 µm, 7.8 x 100 mm, PN: 79547 Resources

Browse app notes, user guides, specification documents, and more in our Knowledge Center.

Browse app notes, user guides, specification documents, and more in our Knowledge Center.

Sugar cubes

Application Note

Analyzing Sweeteners with the HC-75 Ca++ Form

The HC-75 Ca++ form by Hamilton Company easily completes this task. The stationary phase enables quick analysis of both sweetening sugars and sugar alcohols.
Greenhouse gas

Application Note

Bio Fermented Alcohol Detection Using Isocratic Cation Exchange HPLC (HC-75 H+)

Concern over detrimental greenhouse gases produced from the combustion of fossil fuels has been growing for the past few decades.
Beer Fermentation

Application Note

Speed, Resolution, Profit: Why Your Fermentation Analysis Can’t Wait (HC-75 H+)

The examination of fermentation products is an essential attribute for the optimization of biologically made products such as beer, wine, alcohol, and biofuels.

Browse app notes, user guides, specification documents, and more in our Knowledge Center.

cation exchange column

Article

Instructions - HPLC Column Instructions for HC-75 and HC-40 Soft-Gels

Access comprehensive instructions for HC-75 and HC-40 Soft Gel HPLC columns to ensure proper use, maintenance, and optimal performance.
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