HC-75 (H+) Cation Exchange HPLC Column, 4.1 x 250 mm, 9 µm
HC-40 and HC-75 resins are soft gel column packings designed for oligosaccharide separations. The mobile phase of these columns is water without salts or buffer. This simplifies mobile phase preparation, reduces cost, and broadens the use of available detectors. Each of these columns has distinct retention characteristics and is designed for specific separations.
Application Examples: Oligosaccharides (degree of polymerization ≤ 8)
Found in these product categories:
Part/REF # 79476-
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Specifications
Separation Mode |
Separation Mode
Cation Exchange
|
---|---|
Stationary Phase |
Stationary Phase
HC-75 (H+)
|
Format |
Format
HPLC Column
|
Hardware Inner Diameter |
Hardware Inner Diameter
4.1 mm
|
Hardware Length |
Hardware Length
250 mm
|
Particle Size |
Particle Size
9 µm
|
USP Classification |
USP Classification
USP L17
|
Additional specifications
Packing Material Type |
Packing Material Type
PSDVB/Sulfonic Acid
|
---|---|
Pore Size |
Pore Size
Gel-Type
|
Hardware Material |
Hardware Material
Stainless Steel
|
Max Pressure |
Max Pressure
400 psi
|
Exchange Capacity |
Exchange Capacity
5 meq/gm
|
Exchange Capacity/Column |
Exchange Capacity/Column
12 meq/column
|
Mobile Phase Limits |
Mobile Phase Limits
100% Water 0-40% Acetonitrile
|
Buffer Strength |
Buffer Strength
0.0-0.5N
|
Temp Limits |
Temp Limits
25-90°C
|
Restoration |
Restoration
Flush with 0.1 N sulfuric acid at 0.1 mL/min overnight.
|
Compound Classification |
Compound Classification
Carbohydrates
|
UOM |
UOM
1 EACH
|
Resources
Documents
-
Guides
-
SDS
Related Content
- Raffinose Family of Oligosaccharides by Cation Exchange HPLC (HC-75 Ca2+) Application Note
- Bio Fermented Alcohol Detection Using Isocratic Cation Exchange HPLC (HC-75 H+) Application Note
- Saccharide Identification in Honey by Cation Exchange HPLC (HC-75 Pb2+) Application Note
- HPLC Guard Columns, HPLC Guard Cartridges Article
- How Do I Connect a Stainless Steel Guard Column to My HPLC Column? FAQ
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