PRP-X500 7 µm 4.6 x 50 mm, PEEK, PN: 79474

Anion Exchange, PEEK

Part # 79474

PRP-X500 (PN: 79474) is a superficially porous polymeric anion exchange column designed for the separation, purification, and isolation of proteins, peptides, and DNA/RNA.  

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Minimum: 1, Maximum: 1000

PRP-X500 7 µm 4.6 x 50 mm, PEEK, PN: 79474 Details

PRP-X500 is a superficially porous polymeric anion exchange column. The methacrylate polymeric coating of the PRP-X500 provides a more hydrophilic surface, preventing hydrophobic interaction sample losses typically seen on other commercially available protein HPLC columns. Recovery of the sample is excellent with PRP-X500, and the support’s limited permeability prevents proteins from entering the pores and unfolding, which causes peak ghosting. The superficially porous properties of the packing material improve mass transfer, shortening the diffusion path of the analyte. This results in shortened run times, improved resolution, and sharp sample bands.

Application Examples: Proteins, peptides, and DNA/RNA.

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PRP-X500 7 µm 4.6 x 50 mm, PEEK, PN: 79474 Specifications

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Separation Mode Anion Exchange
Stationary Phase PRP-X500
Format HPLC Column
Hardware Inner Diameter 4.6 mm
Hardware Length 50 mm
Particle Size 7 µm
USP Classification USP L23
Packing Material Type Poly(methacryl amido-propyl trimethyl ammonium chloride)
Pore Size Superficially Porous
Particle Surface Area (m2/g) 1.4
Pore Volume (cm3/g) 0.0063
Hardware Material PEEK
Max Pressure 5,000 psi
Exchange Capacity 1.6 meq/gm
Exchange Capacity/Column 1.92 meq/column
Mobile Phase Limits pH 0-9
Buffer Strength 0.0-2.0N
Temp Limits 5-30°C
Compound Classification Large Proteins/Labeled DNA
UOM 1 EACH
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