HC-75 (Pb+2) 9 µm, 7.8 x 300 mm, PN: 79438

Cation Exchange, Stainless steel

Part # 79438

HC-75 is a poly (styrene-divinylbenzene) copolymer with 7.5% cross-linkage.  HC-75 (PN: 79438) is a soft gel column designed for oligosaccharide separations. Each of these columns has distinct retention characteristics and is designed for specific separations. 

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HC-75 (Pb+2) 9 µm, 7.8 x 300 mm, PN: 79438 Details

HC-75 resin in lead (Pb2+) form is a soft gel column packing designed for oligosaccharide separations. The mobile phase of these columns is water without salts or buffer. This simplifies mobile phase preparation, reduces cost, and broadens the use of available detectors. Each of these columns has distinct retention characteristics and is designed for specific separations.

Application Examples: Oligosaccharides (degree of polymerization ≤ 8)

Scientific Citations

HC-75 (Pb+2) 9 µm, 7.8 x 300 mm, PN: 79438 Specifications

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Separation Mode Cation Exchange
Stationary Phase HC-75 (Pb+2)
Format HPLC Column
Hardware Inner Diameter 7.8 mm
Hardware Length 300 mm
Particle Size 9 µm
USP Classification USP L34
Packing Material Type PSDVB/Sulfonic Acid
Pore Size Gel-Type
Hardware Material Stainless Steel
Max Pressure 400 psi
Exchange Capacity 5 meq/gm
Mobile Phase Limits 100% Water 0-40% Acetonitrile
Buffer Strength Water
Temp Limits 25-90°C
Restoration Flush with 1% lead nitrate at 0.1 mL/min overnight.
Compound Classification Carbohydrates
UOM 1 EACH

HC-75 (Pb+2) 9 µm, 7.8 x 300 mm, PN: 79438 Resources

Browse app notes, user guides, specification documents, and more in our Knowledge Center.

Browse app notes, user guides, specification documents, and more in our Knowledge Center.

High Quality Wines

Application Note

Precision You Can Taste: Simplifying Organic Acid Analysis in Wine (HC-75)

The HC-75 H+ Form chromatography resin is specifically engineered for the analysis of six critical fermentation analytes the organic acids (tartaric, malic, lactic, and citric acids) while accurately assessing the key carbohydrates (glucose and glycerol).
Greenhouse gas

Application Note

Bio Fermented Alcohol Detection Using Isocratic Cation Exchange HPLC (HC-75 H+)

Concern over detrimental greenhouse gases produced from the combustion of fossil fuels has been growing for the past few decades.
Saccharide Identification in Honey

Application Note

Saccharide Identification in Honey by Cation Exchange HPLC (HC-75 Pb2+)

Is the honey you consume a prime target for adulteration with refined sugars? Read our app note on saccharide identification.

Browse app notes, user guides, specification documents, and more in our Knowledge Center.

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Instructions - HPLC Column Instructions for HC-75 and HC-40 Soft-Gels

Access comprehensive instructions for HC-75 and HC-40 Soft Gel HPLC columns to ensure proper use, maintenance, and optimal performance.
cation exchange column

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