Sterility Validation of Cell Care FLASK: Closed Fluidic System Enabling Operation Without Laminar Airflow

Cell Care FLASK system with fluidic tubing, caps, and connectors on lab bench; close-up of tube fittings

Sterile Cell Culture Without Laminar Airflow Using Cell Care FLASK

Maintaining sterility is critical in cell culture workflows, yet traditional processes rely heavily on biosafety cabinets and manual handling. This technical note demonstrates how Cell Care FLASK enables sterile, automated T-flask processing through a fully enclosed, chemically decontaminated fluidic system. By eliminating open liquid handling, the system ensures contamination-free operation—even without laminar airflow.

  • Validated sterility performance: Microbial challenge testing confirmed effective decontamination and prevention of carryover contamination.
  • Closed-system processing: Automated workflows maintain sterility without reliance on biosafety cabinets or laminar airflow.
  • Contamination-free cell culture: HEK293 cells remained free of microbial and mycoplasma contamination across multiple passages, with and without antibiotics.

Read the Full Technical Note to Learn More.