Watchmaker RNA Library Prep Kit on Microlab NGS STAR

Application note cover for Watchmaker RNA Library Prep Kit with Polaris Depletion automated on Hamilton Microlab NGS STAR

Automated RNA Library Prep with Polaris® Depletion on the NGS STAR

This technical note presents the automation of the Watchmaker RNA Library Prep Kit with Polaris® Depletion on Hamilton’s Microlab® NGS STAR. Supporting inputs as low as 1 ng total RNA, this streamlined, full-day workflow enables ultra-low input whole transcriptome sequencing (WTS) with high precision and throughput—ideal for degraded or FFPE samples.

Key Takeaways

  • Flexible configurations: Both MOA and LSA platforms support 1–96 samples with consistent library quality, even at ultra-low inputs.
  • Optimized for RNA quality and scale: Polaris® Depletion removes rRNA and globin transcripts for improved transcriptome coverage.
  • Reproducible sequencing performance: Minimal cross-contamination, low CVs, and consistent gene expression profiling validate the automated protocol.

Read the Full Application Note to Learn More.