Automated DNA Library Preparation for Whole Genome Sequencing

Watchmaker DNA Library Prep Kit with Fragmentation Automated on Hamilton Microlab® NGS STAR 

Reliable whole-genome sequencing depends on consistent library quality across varying sample types and throughput demands. This application note demonstrates the automation of the Watchmaker DNA Library Prep Kit with Fragmentation on Hamilton Microlab NGS STAR configurations, providing a flexible workflow designed to reduce hands-on time, minimize variability, and support high-throughput DNA sequencing applications.

Key Takeaways:

• Flexible throughput: Process 1–96 samples per run, generating sequencing-ready libraries in less than 4 hours with the NGS STAR MOA configuration, or less than 7 hours with the NGS STAR LSA configuration.
• Consistent library quality: Automated workflows produced library yield and size comparable to manual preparation, with high-throughput testing demonstrating ≤10% CV in concentration and ≤1% CV in library size.
• Robust performance across sample types: Testing across multiple agriculturally relevant plant, animal, and bacterial species demonstrated reproducible sequencing metrics and strong comparability between automated and manual workflows.

Read the Full Application Note to Learn More.