High-Throughput Production of a Pharmaceutical Protein

Application note 1708 02 Pharma Protein HT Fermentation Cover

High-Throughput Pharmaceutical Protein Production with bioREACTOR 48

Efficient strain screening is critical for recombinant protein development. This application note presents an automated high-throughput fermentation platform combining the Hamilton Microlab® STARlet with the 2mag bioREACTOR 48 to accelerate process optimization using WACKER’s ESETEC® secretion system. The solution enables parallel cultivation of up to 48 E. coli strains with controlled feeding, pH adjustment, induction, and sampling.

  • Parallel process development: Screen up to 48 strains simultaneously under fermenter-like conditions.
  • Improved scalability: Demonstrated one-to-one transferability of optimized conditions from HTF-robot to 1L fermenter scale.
  • Higher productivity vs. shake flasks: Achieved up to 5–8× higher OD600 and 8–10× higher product titers.

Read the full Application Note to learn more.