Automated Affinity and Size Exclusion Chromatography with Protein A and SizeX IMCStips on the Microlab STAR V
Target protein purification and enrichment prior are used prior to HPLC measurements after monoclonal antibody production. Affinity applications for IgG isolation, recombinant protein purification, anti-drug antibody assay, therapeutic drug monitoring provides a mild and reliable separation of interfering impurities. Moreover, the protein analysis workflows often require additional buffer exchange or removal of salts that can interfere with downstream processes and analytical measurements. Therefore, size exclusion chromatography is frequently used.
SizeX IMCStips® are miniaturized affinity and size exclusion pipette tips and are an effective method for protein analysis due to the mild separation conditions used to obtain high-resolution separations. Here, we demonstrate the reproducible and high-yield performance of Protein Affinity followed by SizeX IMCStips on a Hamilton Microlab STAR V liquid handling system by processing cell culture supernatant.
This real-world example at Bayer AG's laboratories shows synergy between IMCS’s chromatography expertise and Hamilton’s robotic prowess maximizes throughput and reproducibility while minimizing human error and operational costs. It exemplifies the power of collaboration in advancing scientific innovation, thus pushing the boundaries of biotechnology and pharmaceuticals,
High reproducibility and yield
Hamilton Microlab STAR V allows high reproducibility and high yield of antibody recoveries after size exclusion chromatography using 8 individual pipetting channels and 96 Multiprobe Head. Hands-free purification and buffer exchange of up to two 96-well plates could be achieved in one run.
Time-Saving Automation
The manual processing of dozens-to-hundreds of protein samples (e.g. for buffer exchange) can be both tedious and error-prone. Microlab STAR V allows high-through put and significantly reduces hands-on time. Automated barcode verification ensures the correct placement of reagents and samples, minimizing errors and streamlining the process. This high level of automation allows laboratories to focus on other critical tasks.
Process Safety over several workflow steps
SizeX IMCStips® automation on Hamilton Microlab STAR V can be readily coupled with additional automated workflows such as multi-attribute method and size exclusion. This saves time and increases process safety.
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