Next Generation Sequencing (NGS)

Powerful Solutions to Invigorate Next Generation Sequencing Methods

Accelerate sequencing with less time on tedious manual library preparation and more time on data analysis. Hamilton automated liquid handling workstations fully automate library preparation workflow steps during next-generation sequencing (NGS) applications.

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Specific Applications

Fully Automated Library Quantification for Illumina® Sequencing on the NGS STAR

In this case study, we describe a newly developed automated method for the quantification of Illumina NGS libraries using the KAPA Library Quantification Kit on the NGS STAR for Library Prep.
TruSight Tumor 170 Automated on the NGS STAR for Illumina Library Prep

Hamilton Robotics and Illumina, Inc have partnered to automate the TruSight Tumor 170 library preparation kit on the NGS STAR. TruSight Tumor 170 simultaneously analyzes DNA and RNA while covering a wide range of genes and variant types. TruSight Tumor 170 is capable of producing accurate results from low-quality formalin-fixed paraffin-embedded (FFPE) samples with a minimum input requirement of 40 ng. The design of the NGS STAR, as a single configuration, enables implementation of additional methods as well as future Illumina protocols using the TruSight Oncology workflow as they are released.
Automation of SOPHiA Clinical Exome Solution on Hamilton STARlet

The BioAnalytica-Genotypos center in Athens chose to couple the Hamilton STARlet robot with the SOPHiA Clinical Exome Solution (CES) to deal with the increasing workload they were facing. In fact, over the past decade, the number of samples and types of analyses processed in the laboratory increased exponentially, leading to pressure to develop, optimize, and validate NGS high-throughput assays. To ensure high-quality results on the new workflow and facilitate its implementation, the automated protocol has been extensively tested by SOPHiA GENETICS and Hamilton on a smaller representative panel of 128 genes. The validation study ensured reliable and flexible automation, which has been efficiently applied on larger gene panels, including CES.
NEOonsite assays automated on the Hamilton NGS STAR

In this application note, we discuss the results and findings from a customer project to automate NEOonsite assays using Hamilton’s NGS STAR workstation, aiming for a reduction of hands-on time and number of user interventions, while keeping a high level of reproducibility.

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Common NGS Kits and Available Hamilton Methods

Are you tired of laborious and inefficient genetic sequencing methods? Say hello to targeted enrichment! This revolutionary technique allows for specific regions of DNA to be isolated and enriched, resulting in more accurate and reliable sequencing data. Our targeted enrichment methods utilize cutting-edge technology to provide you with high-quality results, saving you time, money, and resources. Say goodbye to traditional shotgun sequencing and hello to targeted enrichment - the future of genetic analysis.

Check out some of the targeted enrichment methods we offer:

Legend:
X = Method qualified and developed in cooperation with vendor
X = Method verified by Hamilton and/or internationally renowned customers
X = Method available
* = System configuration available up until May 2023
** = NGS STAR upgrades available from March 2023 onwards

Targeted Enrichment	NGS STARlet	NGS STAR	NGS STAR 96 *	NGS STAR MOA **	NGS STAR V
Agilent SureSelect XT HS2 (DNA and RNA System)		X
Agilent SureSelect XT Low Input Dual Index		X
AmpliSeq for Illumina Cancer Hotspot Panel v2	X
AmpliSeq for Illumina Comprehensive Panel v3	X
AmpliSeq for Illumina Focus Panel	X
IDT xGen Enrichment for Illumina (Exome Research Panel)		X
Illumina AmpliSeq Custom Panels		X
Illumina COVIDSeq		X	X
Illumina DNA Prep					X
Illumina DNA Prep with Enrichment	X	X
Illumina DNA Prep with Exome 2.0 Plus Enrichment	X	X			X
Illumina Nextera XT DNA		X			X
Illumina TruSight Oncology 500	X	X
Illumina TruSight Oncology 500 HT				X
Illumina TruSight RNA Fusion		X
Illumina TruSight Tumor 170	X	X
llumina 16S rRNA Seq		X	X
NEBNext ARTIC SARS-CoV-2 FS Library Prep		X	X
NEBNext Ultra II DNA Library Prep for Illumina					X
NEBNext Ultra II FS DNA Library Prep for Illumina					X
Oxford Nanopore PCR tiling (SARS-CoV-2, ARTIC protocol)			X
Oxford Nanopore PCR tiling of SARS-CoV-2 ('Midnight Kit' version)			X
Paragon Genomics CleanPlex-SARS-CoV-2-Flex-Panel		X
Qiagen QIAseq DIRECT SARS-CoV-2 Kit		X	X
Qiagen QIAseq Multimodal Panel		X
Qiagen QIAseq Multimodal Panel HT		X
Qiagen QIAseq SARS-CoV-2 Primer Panel & Qiagen QIAseq FX DNA		X
Qiagen QIAseq Targeted DNA Panel (multiple panels)		X
Qiagen QIAseq Targeted DNA Pro Panels		X		X
Roche Avenio Tumor Tissue CGP Kit	X	X
Roche KAPA EvoPlus					X
Roche KAPA HyperCap Workflow v3.0		X
Thermo Fisher Scientific AgriSeq HTS Library Kit		X
Twist Bioscience Human Core Exome		X
Twist Bioscience SARS-CoV-2 Research Panel		X	X

Are you searching for a way to gain a complete understanding of an organism's genetic blueprint? Look no further than whole genome sequencing! Our cutting-edge technology and methodologies make it possible to sequence an organism's complete genome, providing a wealth of detailed genetic information.
Our team of experts will work with you to customize the sequencing process to meet your unique research goals, from sample preparation to data analysis. We utilize a variety of methods to ensure the highest quality results, including:

Legend:
X = Method qualified and developed in cooperation with vendor
X = Method verified by Hamilton and/or internationally renowned customers
X = Method available
* = System configuration available up until May 2023
** = NGS STAR upgrades available from March 2023 onwards

Whole Genome Sequencing	NGS STARlet	NGS STAR	NGS STAR 96 *	NGS STAR MOA **	NGS STAR V
Illumina DNA PCR-Free Library Prep, Tagmentation	X	X		X
Illumina DNA Prep		X	X
Illumina DNA Prep with Exome 2.0 Plus Enrichment					X
Illumina Nextera XT		X
Illumina TruSeq DNA PCR-Free		X
Illumina TruSeq Nano DNA		X
NEBNext Enzymatic Methyl-Seq		X
NEBNext Ultra II DNA Library Prep		X
Oxford Nanopore Genomic DNA by Ligation (SQK-LSK109-XL)			X
Oxford Nanopore Genomic DNA by Ligation (SQK-LSK114-XL)	X		X
Oxford Nanopore Genomics Multiplex Ligation Sequencing (SQK-MLK111.96-XL)			X
PacBio Whole Genome and metagenome libraries using SMRTbell prep kit 3.0				X
Perkin Elmer NEXTFLEX Cell Free DNA-Seq Library Prep Kit 2.0		X
QIAseq FX DNA Library Prep	X	X
Roche KAPA HyperPlus		X
Roche KAPA HyperPrep		X
Twist Bioscience Library Preparation EF 2.0 with Enzymatic Fragmentation and Twist Universal Adapter System	X
TWIST Bioscience Exome 2.0 Library Prep with Target Enrichment					X

Want to dive deeper into the genetic makeup of your samples? Transcriptome sequencing is the key! This advanced technique allows you to sequence and analyze the complete set of RNA transcripts in your samples, providing invaluable insight into gene expression and regulation.

Our state-of-the-art transcriptome sequencing services utilize cutting-edge technology to provide you with accurate and reliable data. We offer a range of methods to meet your specific research needs, including:

Legend:
X = Method qualified and developed in cooperation with vendor
X = Method verified by Hamilton and/or internationally renowned customers
X = Method available
* = System configuration available up until May 2023
** = NGS STAR upgrades available from March 2023 onwards

Transcriptome Sequencing	NGS STAR	NGS STAR MOA **	NGS STAR V
Illumina Stranded mRNA Prep and Ligation	X	X
Illumina Stranded Total RNA Prep Ligation with Ribo-Zero Plus	X
Illumina TruSeq Stranded mRNA	X
Illumina TruSeq Stranded Total RNA	X
Lexogen QuantSeq 3´mRNA-Seq Library for Illumina	X
NEBNext Ultra II Directional RNA (Poly(A) mRNA isolation)	X
NEBNext Ultra II Directional RNA (rRNA depletion)	X
Roche KAPA mRNA HyperPrep	X
Roche KAPA RNA HyperPrep	X
Roche KAPA RNA HyperPrep Kit with RiboErase (HMR)			X
Takara SMART-Seq HT	X

Our team of experts will work with you to customize the sequencing process and provide you with high-quality data and comprehensive analysis. With our transcriptome sequencing services, you can unlock a wealth of information about gene expression and regulation, leading to new insights into disease mechanisms and potential therapeutic targets.

Contact us today to learn more about our transcriptome sequencing services and how we can help you advance your research goals.

Advantages of Automating With Hamilton

Massive Time Efficiencies with Hamilton Automated Solutions

Increase sample throughput
Focus on high value activities through hands-free, hassle-free workflows
Eliminate the risk of manual errors and variation

Hamilton utilizes barcode readers to find and identify components on deck

Positively Impact Results with Hamilton Automated Solutions

Easily track samples and reagents via automated barcode scanning
Standardize workflows for consistency and reliability without user bias
Superior measurement accuracy, precision, and reproducibility via air displacement pipetting using Hamilton tips with CO-RE (Compressed O-Ring Expansion) technology
Performance monitoring, even of the most challenging liquids, through Anti-Droplet Control (ADC), Liquid Level Detection (LLD), Total Aspiration and Dispense Monitoring (TADM)

Leverage Top Tier Support with Hamilton Automated Solutions

Adapt to changing lab needs through scalable, modular automated solutions
Reduce internal validation requirements through pre-configured workflows that are qualified to work with industry-leading NGS chemistry providers
Receive personalized recommendations and training from dedicated Hamilton NGS experts

Solutions for Your Science

Explore the many ways in which Hamilton automated workflows benefit NGS applications.

Illumina Solutions
New England Biolabs
Roche-KAPA
PacBio Solutions
Library Validation and Normalization and Pooling

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Molecular Diagnosis Methods - National Health Laboratory Service, South Africa Case Study

In this Case Study, the National Health Laboratory Service (NHLS) Laboratory at Tambo Memorial Hospital in Boksburg, South Africa, describes how they set up a flexible, easy-to-use, high-throughput automated workflow for qPCR-based SARS-CoV-2 testing, using Hamilton (Assay-Ready) Workstations.

Genuity Science Rare Diseases Case Study

In this Case Study, Genuity Science, a privately-funded genomics company, explains the benefits of automating NGS workflows for trio analysis in patients with rare diseases.

Case Study - CeGaT - Next Generation Sequencing Library Prep - NGS in the Field of Precision of Medicine-Oncology

In this case study, CeGaT, an innovative diagnostics company in Germany, explains the benefits of automating the workflows for NGS library preparation for the analyses of Tumor Mutational Burden (TMB) and Microsatellite Instability (MSI).

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