Next-Generation Sequencing (NGS)

Powerful Solutions to Invigorate Next Generation Sequencing Methods
Accelerate sequencing with less time on tedious manual library preparation and more time on data analysis. Hamilton automated liquid handling workstations fully automate library preparation workflow steps during next-generation sequencing (NGS) applications.
Specific Applications
-
Fully Automated Library Quantification for Illumina® Sequencing on the NGS STAR
In this case study, we describe a newly developed automated method for the quantification of Illumina NGS libraries using the KAPA Library Quantification Kit on the NGS STAR for Library Prep. -
TruSight Tumor 170 Automated on the NGS STAR for Illumina Library Prep
Hamilton Robotics and Illumina, Inc have partnered to automate the TruSight Tumor 170 library preparation kit on the NGS STAR. TruSight Tumor 170 simultaneously analyzes DNA and RNA while covering a wide range of genes and variant types. TruSight Tumor 170 is capable of producing accurate results from low-quality formalin-fixed paraffin-embedded (FFPE) samples with a minimum input requirement of 40 ng. The design of the NGS STAR, as a single configuration, enables implementation of additional methods as well as future Illumina protocols using the TruSight Oncology workflow as they are released. -
Automation of SOPHiA Clinical Exome Solution on Hamilton STARlet
The BioAnalytica-Genotypos center in Athens chose to couple the Hamilton STARlet robot with the SOPHiA Clinical Exome Solution (CES) to deal with the increasing workload they were facing. In fact, over the past decade, the number of samples and types of analyses processed in the laboratory increased exponentially, leading to pressure to develop, optimize, and validate NGS high-throughput assays. To ensure high-quality results on the new workflow and facilitate its implementation, the automated protocol has been extensively tested by SOPHiA GENETICS and Hamilton on a smaller representative panel of 128 genes. The validation study ensured reliable and flexible automation, which has been efficiently applied on larger gene panels, including CES. -
NEOonsite assays automated on the Hamilton NGS STAR
In this application note, we discuss the results and findings from a customer project to automate NEOonsite assays using Hamilton’s NGS STAR workstation, aiming for a reduction of hands-on time and number of user interventions, while keeping a high level of reproducibility.
Common NGS Kits and Available Hamilton Methods
-
Targeted Enrichment
Are you tired of laborious and inefficient genetic sequencing methods? Say hello to targeted enrichment! This revolutionary technique allows for specific regions of DNA to be isolated and enriched, resulting in more accurate and reliable sequencing data. Our targeted enrichment methods utilize cutting-edge technology to provide you with high-quality results, saving you time, money, and resources. Say goodbye to traditional shotgun sequencing and hello to targeted enrichment - the future of genetic analysis.
Check out some of the targeted enrichment methods we offer:
Legend:
X = Method qualified and developed in cooperation with vendor
X = Method verified by Hamilton and/or internationally renowned customers
X = Method available
* = System configuration available up until May 2023
** = NGS STAR upgrades available from March 2023 onwardsTargeted Enrichment NGS STARlet NGS STAR NGS STAR 96 * NGS STAR MOA ** NGS STAR V Agilent SureSelect XT HS2 (DNA and RNA System)
XAgilent SureSelect XT Low Input Dual Index
XAmpliSeq for Illumina Cancer Hotspot Panel v2
XAmpliSeq for Illumina Comprehensive Panel v3
X
AmpliSeq for Illumina Focus Panel
XIDT xGen Enrichment for Illumina (Exome Research Panel)
XIllumina AmpliSeq Custom Panels
XIllumina COVIDSeq
XXIllumina DNA Prep
XIllumina DNA Prep with Enrichment
XXIllumina DNA Prep with Exome 2.0 Plus Enrichment
XXXIllumina Nextera XT DNA XXIllumina TruSight Oncology 500
XXIllumina TruSight Oncology 500 HT
XIllumina TruSight RNA Fusion
XIllumina TruSight Tumor 170
XXllumina 16S rRNA Seq
XXNEBNext ARTIC SARS-CoV-2 FS Library Prep
XXNEBNext Ultra II DNA Library Prep for Illumina
XNEBNext Ultra II FS DNA Library Prep for Illumina
XOxford Nanopore PCR tiling (SARS-CoV-2, ARTIC protocol)
XOxford Nanopore PCR tiling of SARS-CoV-2 ('Midnight Kit' version)
XParagon Genomics CleanPlex-SARS-CoV-2-Flex-Panel
XQiagen QIAseq DIRECT SARS-CoV-2 Kit
XXQiagen QIAseq Multimodal Panel
XQiagen QIAseq Multimodal Panel HT
XQiagen QIAseq SARS-CoV-2 Primer Panel & Qiagen QIAseq FX DNA
XQiagen QIAseq Targeted DNA Panel (multiple panels) XQiagen QIAseq Targeted DNA Pro Panels XXRoche Avenio Tumor Tissue CGP Kit XXRoche KAPA EvoPlus XRoche KAPA HyperCap Workflow v3.0 XThermo Fisher Scientific AgriSeq HTS Library Kit XTwist Bioscience Human Core Exome XTwist Bioscience SARS-CoV-2 Research Panel XX -
Whole Genome Sequencing
Are you searching for a way to gain a complete understanding of an organism's genetic blueprint? Look no further than whole genome sequencing! Our cutting-edge technology and methodologies make it possible to sequence an organism's complete genome, providing a wealth of detailed genetic information.
Our team of experts will work with you to customize the sequencing process to meet your unique research goals, from sample preparation to data analysis. We utilize a variety of methods to ensure the highest quality results, including:
Legend:
X = Method qualified and developed in cooperation with vendor
X = Method verified by Hamilton and/or internationally renowned customers
X = Method available
* = System configuration available up until May 2023
** = NGS STAR upgrades available from March 2023 onwardsWhole Genome Sequencing
NGS STARlet NGS STAR NGS STAR 96 * NGS STAR MOA ** NGS STAR V Illumina DNA PCR-Free Library Prep, Tagmentation
XXXIllumina DNA Prep
XXIllumina DNA Prep with Exome 2.0 Plus Enrichment
XIllumina Nextera XT
XIllumina TruSeq DNA PCR-Free
XIllumina TruSeq Nano DNA
XNEBNext Enzymatic Methyl-Seq
XNEBNext Ultra II DNA Library Prep
XOxford Nanopore Genomic DNA by Ligation (SQK-LSK109-XL)
XOxford Nanopore Genomic DNA by Ligation (SQK-LSK114-XL)
XXOxford Nanopore Genomics Multiplex Ligation Sequencing
(SQK-MLK111.96-XL)XPacBio Whole Genome and metagenome libraries using SMRTbell prep kit 3.0
XPerkin Elmer NEXTFLEX Cell Free DNA-Seq Library Prep Kit 2.0 XQIAseq FX DNA Library Prep XXRoche KAPA HyperPlus XRoche KAPA HyperPrep XTwist Bioscience Library Preparation EF 2.0 with Enzymatic Fragmentation
and Twist Universal Adapter SystemXTWIST Bioscience Exome 2.0 Library Prep with Target Enrichment X -
Transcriptome Sequencing
Want to dive deeper into the genetic makeup of your samples? Transcriptome sequencing is the key! This advanced technique allows you to sequence and analyze the complete set of RNA transcripts in your samples, providing invaluable insight into gene expression and regulation.
Our state-of-the-art transcriptome sequencing services utilize cutting-edge technology to provide you with accurate and reliable data. We offer a range of methods to meet your specific research needs, including:
Legend:
X = Method qualified and developed in cooperation with vendor
X = Method verified by Hamilton and/or internationally renowned customers
X = Method available
* = System configuration available up until May 2023
** = NGS STAR upgrades available from March 2023 onwardsTranscriptome Sequencing
NGS STARlet NGS STAR NGS STAR 96 * NGS STAR MOA **
NGS STAR V Illumina Stranded mRNA Prep and Ligation
XXIllumina Stranded Total RNA Prep Ligation with Ribo-Zero Plus
XIllumina TruSeq Stranded mRNA
XIllumina TruSeq Stranded Total RNA
XLexogen QuantSeq 3´mRNA-Seq Library for Illumina
XNEBNext Ultra II Directional RNA (Poly(A) mRNA isolation)
XNEBNext Ultra II Directional RNA (rRNA depletion)
XRoche KAPA mRNA HyperPrep
XRoche KAPA RNA HyperPrep
XRoche KAPA RNA HyperPrep Kit with RiboErase (HMR)
XTakara SMART-Seq HT XOur team of experts will work with you to customize the sequencing process and provide you with high-quality data and comprehensive analysis. With our transcriptome sequencing services, you can unlock a wealth of information about gene expression and regulation, leading to new insights into disease mechanisms and potential therapeutic targets.
Contact us today to learn more about our transcriptome sequencing services and how we can help you advance your research goals.
Advantages of Automating With Hamilton

Massive Time Efficiencies with Hamilton Automated Solutions
- Increase sample throughput
- Focus on high value activities through hands-free, hassle-free workflows
- Eliminate the risk of manual errors and variation

Positively Impact Results with Hamilton Automated Solutions
- Easily track samples and reagents via automated barcode scanning
- Standardize workflows for consistency and reliability without user bias
- Superior measurement accuracy, precision, and reproducibility via air displacement pipetting using Hamilton tips with CO-RE (Compressed O-Ring Expansion) technology
- Performance monitoring, even of the most challenging liquids, through Anti-Droplet Control (ADC), Liquid Level Detection (LLD), Total Aspiration and Dispense Monitoring (TADM)

Leverage Top Tier Support with Hamilton Automated Solutions
- Adapt to changing lab needs through scalable, modular automated solutions
- Reduce internal validation requirements through pre-configured workflows that are qualified to work with industry-leading NGS chemistry providers
- Receive personalized recommendations and training from dedicated Hamilton NGS experts

Solutions for Your Science
Explore the many ways in which Hamilton automated workflows benefit NGS applications.
- Illumina Solutions
- New England Biolabs
- Roche-KAPA
- PacBio Solutions
- Library Validation and Normalization and Pooling
Get your Case Study now!
Molecular Diagnosis Methods - National Health Laboratory Service, South Africa Case Study
Genuity Science Rare Diseases Case Study
Case Study - CeGaT - Next Generation Sequencing Library Prep - NGS in the Field of Precision of Medicine-Oncology

Hamilton is Customer Service
Whether you need a new instrument or support for one already in the lab, Hamilton is here to support you. Dedicated staff around the world are ready to respond to your request.
Contact Us