Powerful Solutions to Invigorate Next Generation Sequencing Methods

Accelerate sequencing with less time on tedious manual library preparation and more time on data analysis. Hamilton automated liquid handling workstations fully automate library preparation workflow steps during next-generation sequencing (NGS) applications.


Specific Applications

  • Fully Automated Library Quantification for Illumina® Sequencing on the NGS STAR

    In this case study, we describe a newly developed automated method for the quantification of Illumina NGS libraries using the KAPA Library Quantification Kit on the NGS STAR for Library Prep.
  • TruSight Tumor 170 Automated on the NGS STAR for Illumina Library Prep

    Hamilton Robotics and Illumina, Inc have partnered to automate the TruSight Tumor 170 library preparation kit on the NGS STAR. TruSight Tumor 170 simultaneously analyzes DNA and RNA while covering a wide range of genes and variant types. TruSight Tumor 170 is capable of producing accurate results from low-quality formalin-fixed paraffin-embedded (FFPE) samples with a minimum input requirement of 40 ng. The design of the NGS STAR, as a single configuration, enables implementation of additional methods as well as future Illumina protocols using the TruSight Oncology workflow as they are released.
  • Automation of SOPHiA Clinical Exome Solution on Hamilton STARlet

    The BioAnalytica-Genotypos center in Athens chose to couple the Hamilton STARlet robot with the SOPHiA Clinical Exome Solution (CES) for dealing with the increasing workload they were facing. In fact, over the past decade, the number of samples and types of analyses processed in the laboratory increased exponentially, leading to a pressure to develop, optimize, and validate NGS high-throughput assays. To ensure high-quality results on the new workflow and facilitate its implementation, the automated protocol has been extensively tested by SOPHiA GENETICS and Hamilton on a smaller representative panel of 128 genes. The validation study ensured a reliable and flexible automation, which has been efficiently applied on larger gene panels, including CES.
  • NEOonsite assays automated on the Hamilton NGS STAR

    In this application note, we discuss the results and findings from a customer project to automate NEOonsite assays using Hamilton’s NGS STARTM workstation, aiming for a reduction of hands-on time and number of user interventions, while keeping a high level of reproducibility.

Common NGS Kits and Available Hamilton Methods

Library Prep Kit Method Availability Platform
Agilent SureSelect XT Method available NGS STAR
IDT xGen Enrichment Method qualified by IDT NGS STAR
illumina TruSight Oncology 500Method qualified by illuminaNGS STAR
illumina TruSight Oncology 500HTMethod qualified by illuminaNGS STAR MOA for 500HT
illumina 16S rRNA Method qualified by illumina NGS STAR
illumina Nextera DNA Flex Method in development NGS STAR
illumina Nextera Rapid Capture Method available NGS STAR
illumina Nextera XT DNA Method qualified by illumina NGS STAR
illumina TruSeq DNA PCR-Free Method available NGS STAR with ODTC
illumina TruSeq Nano DNA Method qualified by illumina NGS STAR
illumina TruSeq RNA Exome Method qualified by illumina NGS STAR
illumina TruSeq Stranded mRNA Method qualified by illumina NGS STAR
illumina TruSeq Stranded Total RNA Method qualified by illumina NGS STAR
illumina TruSight Cardio Method available NGS STAR
illumina TruSight One Method available NGS STAR
illumina TruSight RNA Fusion Method available NGS STAR
illumina TruSight Rapid Capture Method available NGS STAR
illumina TruSight Tumor 170 Method qualified by Illumina NGS STAR
KAPA Library Quantification Kit for illuminaMethod qualified by RocheNGS STAR
Roche NimbleGen Target Capture Method qualified by Roche NGS STAR

Roche/KAPA HyperPrep

Method qualified by RocheNGS STAR
Roche/KAPA HyperPlus Method qualified by Roche NGS STAR
Roche/KAPA HyperCap Method qualified by Roche NGS STAR

Advantages of Automating With Hamilton

Massive Time Efficiencies with Hamilton Automated Solutions

  • Increase sample throughput
  • Focus on high value activities through hands-free, hassle-free workflows
  • Eliminate the risk of manual errors and variation

Positively Impact Results with Hamilton Automated Solutions

  • Easily track samples and reagents via automated barcode scanning
  • Standardize workflows for consistency and reliability without user bias
  • Superior measurement accuracy, precision, and reproducibility via air displacement pipetting using Hamilton tips with CO-RE (Compressed O-Ring Expansion) technology
  • Performance monitoring, even of the most challenging liquids, through Anti-Droplet Control (ADC), Liquid Level Detection (LLD), Total Aspiration and Dispense Monitoring (TADM)

Leverage Top Tier Support with Hamilton Automated Solutions

  • Adapt to changing lab needs through scalable, modular automated solutions
  • Reduce internal validation requirements through pre-configured workflows that are qualified to work with industry-leading NGS chemistry providers
  • Receive personalized recommendations and training from dedicated Hamilton NGS experts
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Solutions for Your Science

Explore the many ways in which Hamilton automated workflows benefit NGS applications.

  • Illumina Solutions
  • New England Biolabs
  • Roche-KAPA
  • PacBio Solutions
  • Library Validation and Normalization and Pooling

Hamilton is Customer Service

Whether you need a new instrument or support for one already in the lab, Hamilton is here to support you. Dedicated staff around the world are ready to respond to your request.

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