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The Premiere Solution for NGS

The NGS STAR for Library Prep assay ready workstation is a comprehensive solution for next-generation sequencing (NGS) applications from whole genome to whole transcriptome sequencing and targeted sequencing applications. Fully automated library preparation can be optimized for use with assay chemistries from leading industry partners, with throughputs up to 96 sample libraries processed at once.

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Features and Benefits

Fully Optimized NGS Solution

The NGS STAR for Library Prep is ideal for multiple automated NGS library preparations. Each NGS application is optimized and qualified in collaboration with industry-leading partners such as:

  • Illumina
  • Roche-KAPA
  • PacBio
  • IDT
  • Twist
  • Thermo Fisher Scientific
  • New England BioLabs

Have specific questions, or don’t see a desired provider on this list? Contact our dedicated application experts, who will provide
high quality guidance and translate specific needs into reliable and efficient automated workflows.

Individualized Flexibility

The NGS STAR for Library Prep uses an open deck layout so that every system is fine-tuned to the lab’s specific NGS workflow, throughput and assay needs. At the same time, the system is easily updated to keep pace with protocol updates, or as requirements evolve over time.

Precise, Repeatable Liquid Handling

Air displacement pipetting using Hamilton tips with CO-RE (Compressed O-Ring Expansion) technology provides superior measurement accuracy, precision, and reproducibility without tip distortion or aerosol generation during tip pick-up and ejection.

Hands-Free, Hassle-Free Operation

As a fully walkaway system, NGS STAR for Library Prep significantly reduces cumbersome and user- or sample-variable preparation steps during runs of 1 to 96 samples. User-friendly software guides operators through the automated process, and automated barcode verification ensures proper placement of samples, reagents, plates and tips. Each aspirate and dispense step is dynamically tracked, for confidence that the indexed and multiplexed samples are processed according to the worklist and highly reproducible, even across different sites.


All specifications are based on the Microlab STAR.

Length31.2 in (795 mm)
Width65.6 in (1664 mm)
Height35.6 in (903 mm)

Common NGS Library Prep Kits and Available Hamilton Methods

Library Prep Kit Method Availability
Agilent SureSelect XT Method available
IDT xGen Enrichment Method qualified by IDT
illumina 16S rRNA Method qualified by illumina
illumina Nextera DNA Flex Method in development
illumina Nextera Rapid Capture Method available
illumina Nextera XT DNA Method qualified by illumina
illumina Nextera Flex for EnrichmentMethod qualified by illumina
illumina TruSeq DNA PCR-Free Method available
illumina TruSeq Nano DNA Method qualified by illumina
illumina TruSeq RNA Exome Method qualified by illumina
illumina TruSeq Stranded mRNA Method qualified by illumina
illumina TruSeq Stranded Total RNA Method qualified by illumina
illumina TruSight Cardio Method available
illumina TruSight Oncology 500Method qualified by illumina
illumina TruSight Oncology 500HTMethod qualified by illumina
illumina TruSight One Method available
illumina TruSight RNA Fusion Method available
illumina TruSight Rapid Capture Method available
illumina TruSight Tumor 170 Method qualified by illumina
KAPA Library Quantification Kit for IlluminaMethod qualified by Roche
Roche NimbleGen Target Capture Method qualified by Roche
Roche/KAPA HyperPrepMethod qualified by Roche
Roche/KAPA HyperPlus Method qualified by Roche
Roche/KAPA HyperCap Method qualified by Roche

Additional Resources for the NGS STAR for Library Prep

See all Hamilton Robotics Automated Liquid Handlers
View all of Hamilton's Assay Ready Workstations
Check out the Hamilton Microlab STAR automated liquid handler
Learn more about the AutoLys STAR

Specific Applications

  • Fully Automated Library Quantification for Illumina® Sequencing on the NGS STAR

    In this case study, we describe a newly developed automated method for the quantification of Illumina NGS libraries using the KAPA Library Quantification Kit on the NGS STAR for Library Prep.
  • TruSight Tumor 170 Automated on the NGS STAR for Illumina Library Prep

    Hamilton Robotics and Illumina, Inc have partnered to automate the TruSight Tumor 170 library preparation kit on the NGS STAR. TruSight Tumor 170 simultaneously analyzes DNA and RNA while covering a wide range of genes and variant types. TruSight Tumor 170 is capable of producing accurate results from low-quality formalin-fixed paraffin-embedded (FFPE) samples with a minimum input requirement of 40 ng. The design of the NGS STAR, as a single configuration, enables implementation of additional methods as well as future Illumina protocols using the TruSight Oncology workflow as they are released.

Hamilton is Customer Service

Whether you need a new instrument or support for one already in the lab, Hamilton is here to support you. Dedicated staff around the world are ready to respond to your request.

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