ONT PCR Tiling of SARS-CoV-2 with Rapid Barcoding and Midnight RT-PCR

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Application note 2409 11 ONT Midnight TIMG

Sequence-based surveillance of circulating pathogens provides important information to understand geographical spreading and transmission networks, as well as to identify and track emerging variants. Not only does this data help to discover mechanisms of viral adaptation and evolution but can also be used to assess vaccine efficacy or inform new vaccine formulations.

During the ongoing SARS-CoV-2 pandemic, genomic epidemiology has become an indispensable tool in the global effort to fight disease outbreaks and emerging resistances.
Oxford Nanopore sequencing has been pivotal in enabling global surveillance, from high-throughput laboratories to decentralized regional approaches. This makes the rapid identification of newly emerging variants possible, while operating cost-effectively and with high-quality standards at the same time.

Sequencing the SARS-CoV-2 genome with the Midnight protocol offers a simple workflow with rapid turnaround time. The application enables the processing of a broad range of samples and provides future-ready robustness with timely updates. Automation on the NGS STAR 96 provides the power to increase genomic pathogen surveillance capacities to inform public health.

  • Rapid turnaround time from sample-to-result
  • Flexible sample batching and scalability from small sample numbers to high-throughput needs
  • Updated all-in-one reagent bundle for current and future virus variant

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