HC-75 (H+) 9 µm, 7.8 x 300 mm, PN: 79544

Cation Exchange, Stainless steel

Part # 79544

HC-75 is a poly (styrene-divinylbenzene) copolymer with 7.5% cross-linkage. HC-75 (PN: 79544) is a soft gel column designed for oligosaccharide separations. Each of these columns has distinct retention characteristics and is designed for specific separations. 

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HC-75 (H+) 9 µm, 7.8 x 300 mm, PN: 79544 Details

HC-75 is a poly (styrene-divinylbenzene) copolymer with 7.5% cross-linkage.  The HC-75 column in hydrogen (H+) form is excellent in the separation of sugars in combination with organic acids, alcohols, and glycerol, which is essential in QC and monitoring tests for fuel ethanol fermentation analysis.  The mobile phase is only water with an acid modifier if needed.  This reduces cost, and broadens the use of available detectors.  

Application Examples: Oligosaccharides (degree of polymerization ≤ 8)

Scientific Citations

HC-75 (H+) 9 µm, 7.8 x 300 mm, PN: 79544 Specifications

Print
Separation Mode Cation Exchange
Stationary Phase HC-75 (H+)
Format HPLC Column
Hardware Inner Diameter 7.8 mm
Hardware Length 300 mm
Particle Size 9 µm
USP Classification USP L17
Packing Material Type PSDVB/Sulfonic Acid
Pore Size Gel-Type
Hardware Material Stainless Steel
Max Pressure 400 psi
Exchange Capacity 5 meq/gm
Exchange Capacity/Column 32 meq/column
Mobile Phase Limits 100% Water 0-40% Acetonitrile
Buffer Strength 0.0-0.5N
Temp Limits 25-90°C
Restoration Flush with 0.1 N sulfuric acid at 0.1 mL/min overnight.
Compound Classification Carbohydrates
UOM 1 EACH

HC-75 (H+) 9 µm, 7.8 x 300 mm, PN: 79544 Resources

Browse app notes, user guides, specification documents, and more in our Knowledge Center.

Browse app notes, user guides, specification documents, and more in our Knowledge Center.

Saccharide Identification in Honey

Application Note

Saccharide Identification in Honey by Cation Exchange HPLC (HC-75 Pb2+)

Is the honey you consume a prime target for adulteration with refined sugars? Read our app note on saccharide identification.
High Quality Wines

Application Note

Precision You Can Taste: Simplifying Organic Acid Analysis in Wine (HC-75)

The HC-75 H+ Form chromatography resin is specifically engineered for the analysis of six critical fermentation analytes the organic acids (tartaric, malic, lactic, and citric acids) while accurately assessing the key carbohydrates (glucose and glycerol).
Sugar cubes

Application Note

Analyzing Sweeteners with the HC-75 Ca++ Form

The HC-75 Ca++ form by Hamilton Company easily completes this task. The stationary phase enables quick analysis of both sweetening sugars and sugar alcohols.

Browse app notes, user guides, specification documents, and more in our Knowledge Center.

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