What does this white paper cover?

This white paper validates that direct measurement of the Microlab 600 syringe drive stem displacement can accurately predict dispense volumes, serving as a reliable method for instrument calibration across syringe sizes from 10 μL to 50 mL.

What factors affect dispense accuracy on the Microlab 600?

Dispense accuracy is determined by two factors: the inner diameter of the syringe barrel and the positional accuracy of the syringe drive. The Microlab 600 is designed to compensate for allowable manufacturing tolerances in syringe inner diameter.

How was the testing conducted?

Ten 1 mL syringes were measured for inner barrel diameter, and linear drive stem displacement was recorded at 1%, 5%, and 30% stroke. Predicted dispense volumes were then compared against gravimetrically measured volumes using deionized water, with each measurement repeated ten times.

Did the Microlab 600 meet its accuracy specifications during testing?

Yes. Across all stroke levels and all ten syringes tested, both predicted and gravimetrically measured dispense volumes fell within the published Microlab 600 accuracy specifications.

Should users test the Microlab 600 with their own solvents?

Yes. Hamilton recommends testing the Microlab 600 under real laboratory conditions using the actual solvents to be dispensed, as factors like solvent compressibility, atmospheric pressure, and humidity can influence dispense results.

Microlab 600 Dispense Accuracy: Predicted vs. Gravimetric

This white paper examines how the Microlab 600 syringe pump's positional accuracy can be used to reliably predict dispense volumes across a range of syringe sizes, from 10 μL to 50 mL. It explains how two variables, syringe inner diameter and drive stem displacement, work together to determine dispense accuracy, and why the Microlab 600's precision is sufficient to compensate for allowable manufacturing tolerances in syringe barrel diameter.

The document walks through the full testing methodology, including inner diameter measurements taken from ten 1 mL syringes, linear displacement measurements at 1%, 5%, and 30% stroke, and a side-by-side comparison of predicted versus gravimetrically measured dispense volumes. Results across all stroke levels fell within Microlab 600 specifications, validating direct drive stem measurement as a reliable calibration method. The conclusion also offers practical guidance on testing under real laboratory conditions with the actual solvents being dispensed.

Microlab 600 Predicted Dispense Volume vs Gravimetric Measurement

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