Dissolved CO₂ (pCO₂) at the Bioreactor
Why is real‑time pCO₂ monitoring critical for stable, scalable bioprocesses?
Dissolved carbon dioxide (pCO₂) is a Critical Process Parameter (CPP) in biopharma production processes. It directly impacts product titer and critical quality attributes, and influences other culture parameters such as extracellular and intracellular pH, as well as key performance indicators such as Viable Cell Density.
Uncontrolled dissolved carbon dioxide levels in the bioreactor can result in growth inhibition, lower product titer, and decreased product quality. During scale‑up, pCO₂ can accumulate rapidly and remain undetected, introducing variability and limiting process performance.
Why should you download our white paper?
Download our white paper to understand why dissolved carbon dioxide (pCO₂) must be treated as a Critical Process Parameter (CPP) in bioprocesses and how inadequate pCO₂ control limits process performance, reproducibility, and scalability.
In this white paper, you will learn:
- How dissolved CO₂ accumulates in bioreactors and why pCO₂ control becomes more difficult during scale‑up
- How pCO₂ influences pH buffering, cellular metabolism, viable cell density, and product quality attributes
- Why indirect or off‑line CO₂ measurements fail to capture real process dynamics
- How real‑time pCO₂ monitoring supports PAT and Quality‑by‑Design (QbD) strategies in upstream bioprocessing
- How improved pCO₂ control reduces batch‑to‑batch variability across R&D and manufacturing scales
Process Risks Associated With Uncontrolled Dissolved CO₂
Impact on Product Titer
Dissolved CO₂ accumulation can inhibit cell growth and metabolic activity. Our white paper explains how elevated pCO₂ influences productivity and why real‑time monitoring is required to understand its effect on product titer during bioprocesses.
Batch-to-Batch Reproducibility
Variations in pCO₂ introduce hidden process variability. Our white paper discusses why treating dissolved CO₂ as a Critical Process Parameter (CPP) is essential for achieving consistent and reproducible bioreactor performance.
Consistency Across R&D and Production Scale Bioreactors
CO₂ stripping and accumulation behave differently at different scales. Our white paper explains how pCO₂‑related scale effects contribute to process deviations and why real‑time monitoring supports more reliable scale‑up and tech transfer.
Download Our White Paper
Understand how PAT and Quality‑by‑Design (QbD) principles help reveal, understand, and control dissolved CO₂.
