Silica packing material for a variety of reversed-phase separations Hamilton HxSil C8 columns are well end-capped for retention time reproducibility and durability. The C8 demonstrates excellent selectivity needed for complex sample mixtures and gives shorter retention times than a C18.
Application Examples: Polycyclic aromatic hydrocarbons, small organic molecules.
Alkylated silica is used for reversed-phase HPLC columns. Hamilton offers two varieties of this composition: C18 and C8. C18 columns are more hydrophobic than C8 columns and thereby retain hydrophobic analytes more strongly than a C8 column will. Due to stronger retention, C18 columns tend to result in longer retention times and better separations. C8 columns result in shorter analyte retention times which produce faster analysis times.
How do I restore my HPLC column?Performance will inevitably degrade as the column is used, however this procedure may restore the performance of a column that has become fouled. Learn more in this step-by-step guide.
How Do I Connect a Stainless Steel Guard Cartridge to My HPLC Column?The process of connecting a guard cartridge to an HPLC column is fairly simple, but requires different steps based on the HPLC instrument and the guard cartridge configuration. Click to read our step-by-step guide.
How Do I Install My HPLC Column?Connecting an chromatography column is not difficult, but if the proper steps aren't followed, chromatographic results can be compromised. Follow this step-by-step guide to get it done right.
USP "L" Column ListingsExplore Hamilton’s qualifying USP “L” columns.
HPLC Guard Columns, HPLC Guard CartridgesHPLC guard columns and guard cartridges protect analytical, semi-prep and preparative HPLC columns. They help by removing particulate contaminants and highly absorptive compounds from samples, prolonging column life.
Work with R&D engineers to customize Hamilton parts
Hamilton's "Custom Built" program puts you directly in contact with a Hamilton R&D Engineer to discuss your application and product needs. The engineer will point you to a standard solution or propose a customized solution to meet your needs.Talk to an engineer