PRP-h5
Pentafluoro reversed-phase column for unique selectivity
The PRP-h5 derives its hydrophobic functionality from a pentafluorinated polymer bead, delivering a selectivity difference from standard silica C18 stationary phases. This gives chromatographers the desired retention characteristics necessary for certain sample types. This difference is especially pronounced for halogenated solutes.
Unlike silica-based C8 or C18 columns, PRP-h5 has no stationary phase coating. Since there is no stationary phase to hydrolyze, the column maintains its performance characteristics longer than silica-based C8 or C18 columns.
Application Examples: Organic compounds: macromolecules (> 2,000 mw), pharmaceuticals, protein digests, tryptic digests, proteomics
-
PRP-h5 Columns
-
PRP-h5 Guard Columns
-
PRP-h5 Bulk Resin
PRP-h5 Overview
Pentafluoro reversed-phase column for unique selectivity The PRP-h5 utilizes the PRP-3 as its base with a pentafluorinated modification, making it more hydrophobic in nature. The PRP-h5, with its functionality derived from a pentafluorinated polymer bead, delivers a selectivity difference from standard silica C18 stationary phases. This gives chromatographers the desired retention characteristics necessary for certain sample types. This difference is especially pronounced for halogenated solutes.
Unlike silica-based C8 or C18 columns, PRP-h5 has no stationary phase coating. Since there is no stationary phase to hydrolyze, the column maintains its performance characteristics longer than many silica-based C8 or C18 columns.
Applications
Organic compounds: macromolecules (> 2,000 mw), pharmaceuticals, protein digests, tryptic digests, proteomics
How to Choose Column Specifications for Your Application
PRP-h5 Applications
Tech Center
Resources
-
Reference Guide - HPLC Columns
Learn how to choose a column, how to maximize the life of a column through proper care, cleaning, and restoration. -
Datasheet - PRP-h5
See how our polymer column enhances sample recovery.
-
How do I restore my HPLC column?
Performance will inevitably degrade as the column is used, however this procedure may restore the performance of a column that has become fouled. Learn more in this step-by-step guide. -
How Do I Connect a Stainless Steel Guard Cartridge to My HPLC Column?
The process of connecting a guard cartridge to an HPLC column is fairly simple, but requires different steps based on the HPLC instrument and the guard cartridge configuration. Click to read our step-by-step guide. -
How Do I Install My HPLC Column?
Connecting an chromatography column is not difficult, but if the proper steps aren't followed, chromatographic results can be compromised. Follow this step-by-step guide to get it done right.
-
USP "L" Column Listings
Explore Hamilton’s qualifying USP “L” columns. -
HPLC Guard Columns, HPLC Guard Cartridges
HPLC guard columns and guard cartridges protect analytical, semi-prep and preparative HPLC columns. They help by removing particulate contaminants and highly absorptive compounds from samples, prolonging column life.
Work with R&D engineers to customize Hamilton parts
Hamilton's "Custom Built" program puts you directly in contact with a Hamilton R&D Engineer to discuss your application and product needs. The engineer will point you to a standard solution or propose a customized solution to meet your needs.
Talk to an engineer