PRP-h5
Pentafluoro reversed-phase column for unique selectivity
The PRP-h5 derives its hydrophobic functionality from a pentafluorinated polymer bead, delivering a selectivity difference from standard silica C18 stationary phases. This gives chromatographers the desired retention characteristics necessary for certain sample types. This difference is especially pronounced for halogenated solutes.
Unlike silica-based C8 or C18 columns, PRP-h5 has no stationary phase coating. Since there is no stationary phase to hydrolyze, the column maintains its performance characteristics longer than silica-based C8 or C18 columns.
Application Examples: Organic compounds: macromolecules (> 2,000 mw), pharmaceuticals, protein digests, tryptic digests, proteomics
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PRP-h5 Columns
PRP-h5 Guard Columns
PRP-h5 Bulk Resin
PRP-h5 Overview
Pentafluoro reversed-phase column for unique selectivity The PRP-h5 utilizes the PRP-3 as its base with a pentafluorinated modification, making it more hydrophobic in nature. The PRP-h5, with its functionality derived from a pentafluorinated polymer bead, delivers a selectivity difference from standard silica C18 stationary phases. This gives chromatographers the desired retention characteristics necessary for certain sample types. This difference is especially pronounced for halogenated solutes.
Unlike silica-based C8 or C18 columns, PRP-h5 has no stationary phase coating. Since there is no stationary phase to hydrolyze, the column maintains its performance characteristics longer than many silica-based C8 or C18 columns.
Applications
Organic compounds: macromolecules (> 2,000 mw), pharmaceuticals, protein digests, tryptic digests, proteomics
How to Choose Column Specifications for Your Application
PRP-h5 Applications
Tech Center
Resources
Hamilton's knowledge base provides a range of explanatory articles, frequently asked questions, and document downloads.-
Reference Guide - HPLC Columns
Learn how to choose a column, how to maximize the life of a column through proper care, cleaning, and restoration.
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How do I restore my HPLC column?
HPLC column chromatography performance will inevitably degrade as the column is used, however this procedure may restore the performance of a column that has become fouled. Click the headline above to read this step-by-step guide. -
How Do I Connect a Stainless Steel Guard Cartridge to My HPLC Column?
The process of connecting a guard cartridge to an HPLC column is fairly simple, but requires different steps based on the HPLC instrument and the guard cartridge configuration. Click the headline above to read this step-by-step guide. -
How Do I Install My HPLC Column?
Connecting an HPLC column is not difficult, but if the proper steps aren't followed, chromatographic results can be compromised. Click the headline above to read this step-by-step guide.
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HPLC Guard Columns, HPLC Guard Cartridges
HPLC guard columns and guard cartridges protect analytical, semi-prep and preparative HPLC columns. They help by removing particulate contaminants and highly absorptive compounds from samples, prolonging column life.
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