PRP-1
PRP-1 columns have inherent reversed-phase separation qualities that eliminate the requirement for a stationary phase coating. Hydrolysis of the stationary phase coating is often the limiting factor in column lifetime, so the lack of coating on the PRP-1 resin extends the longevity of the column. PRP-1 also has exceptional sample recovery (≥ 95%).
(USP L21)
Application Examples: Organic compounds: small molecules (< 2,000 mw), pharmaceuticals, steroids, nucleic acids, vitamins, herbicides.
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PRP-1 Overview
Superior Sample Recovery
Sample recovery is vital to sample purification. Problems arise when labile samples become irreversibly bound to the silanol groups present on C8 and C18 HPLC columns. Since Hamilton polymers are made entirely of polystyrene-divinylbenzene, there are no silanol groups to cause sample loss. Recovery and quantitation of labile and reactive samples is enhanced. The purification of protected oligonucleotides demonstrates the enhanced recovery of polymer supports. While approximately 50–80% of an oligonucleotide is recovered on a C18 column, the equivalent PRP-1 column recovers 95% or greater of the same sample.
Unlike silica-based C8 or C18 columns, PRP-1 has no stationary phase coating. The integral reversed-phase characteristics of the PRP-1 column eliminate the need for special coating techniques. Since there is no stationary phase to hydrolyze, the column maintains its performance characteristics longer than many C8 or C18 columns.
Applications:
Organic compounds: small molecules (< 2,000 mw), pharmaceuticals, steroids, nucleic acids, vitamins, herbicides
Examples of analytes that can be separated on PRP-1 columns:
- Polycyclic aromatic hydrocarbons (PAH)
- Ionizable organic compounds
- Steroids
- Peptide fragments
Tech Center
PRP-1 Resources
Browse app notes, user guides, specification documents, and more in our Knowledge Center.
Browse app notes, user guides, specification documents, and more in our Knowledge Center.
Browse app notes, user guides, specification documents, and more in our Knowledge Center.
Browse app notes, user guides, specification documents, and more in our Knowledge Center.
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