Hamilton PRP-1 HPLC Column

PRP-1 columns have inherent reversed-phase separation qualities that eliminate the requirement for a stationary phase coating. Hydrolysis of the stationary phase coating is often the limiting factor in column lifetime, so the lack of coating on the PRP-1 resin extends the longevity of the column. PRP-1 also have exceptional sample recovery (≥ 95%).

(USP L21)

Application Examples: Organic compounds: small molecules (< 2,000 mw), pharmaceuticals, steroids, nucleic acids, vitamins, herbicides.

  • PRP-1 Columns

  • PRP-1 Guard Columns

  • PRP-1 Bulk Resin

PRP-1 Overview

Superior Sample Recovery

Sample recovery is vital to sample purification. Problems arise when labile samples become irreversibly bound to the silanol groups present on C8 and C18 HPLC columns. Since Hamilton polymers are made entirely of poly styrene-divinylbenzene, there are no silanol groups to cause sample loss. Recovery and quantitation of labile and reactive samples is enhanced. The purification of protected oligonucleotides demonstrates the enhanced recovery of polymer supports. While approximately 50–80% of an oligonucleotide is recovered on a C18 column, the equivalent PRP-1 column recovers 95% or greater of the same sample.

Unlike silica-based C8 or C18 columns, PRP-1 has no stationary phase coating. The integral reversed-phase characteristics of the PRP-1 column eliminate the need for special coating techniques. Since there is no stationary phase to hydrolyze, the column maintains its performance characteristics longer than many C8 or C18 columns.


Organic compounds: small molecule (< 2,000 mw), pharmaceuticals, steroids, nucleic acids, vitamins, herbicides

Examples of analytes that can be separated on PRP-1 columns:

How to Choose Column Specifications for Your Application

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Specific Applications


Hamilton's knowledge base provides a range of explanatory articles, frequently asked questions, and document downloads.
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