PRP-1

PRP-1 columns have inherent reversed-phase separation qualities that eliminate the requirement for a stationary phase coating. Hydrolysis of the stationary phase coating is often the limiting factor in column lifetime, so the lack of coating on the PRP-1 resin extends the longevity of the column. PRP-1 also have exceptional sample recovery (≥ 95%).
(USP L21)
Application Examples: Organic compounds: small molecules (< 2,000 mw), pharmaceuticals, steroids, nucleic acids, vitamins, herbicides.
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PRP-1 Columns
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PRP-1 Guard Columns
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PRP-1 Bulk Resin
PRP-1 Overview
Superior Sample Recovery
Sample recovery is vital to sample purification. Problems arise when labile samples become irreversibly bound to the silanol groups present on C8 and C18 HPLC columns. Since Hamilton polymers are made entirely of poly styrene-divinylbenzene, there are no silanol groups to cause sample loss. Recovery and quantitation of labile and reactive samples is enhanced. The purification of protected oligonucleotides demonstrates the enhanced recovery of polymer supports. While approximately 50–80% of an oligonucleotide is recovered on a C18 column, the equivalent PRP-1 column recovers 95% or greater of the same sample.
Unlike silica-based C8 or C18 columns, PRP-1 has no stationary phase coating. The integral reversed-phase characteristics of the PRP-1 column eliminate the need for special coating techniques. Since there is no stationary phase to hydrolyze, the column maintains its performance characteristics longer than many C8 or C18 columns.
Applications:
Organic compounds: small molecule (< 2,000 mw), pharmaceuticals, steroids, nucleic acids, vitamins, herbicides
Examples of analytes that can be separated on PRP-1 columns:
How to Choose Column Specifications for Your Application
Tech Center
Specific Applications
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Allergens Contained in Balsam of Peru (PRP-C18 / PRP-1)
In some cases it has been shown that allergies can arise from some of the individual components of the essential oil Balsam of Peru. Read our app note detailing an HPLC method that separates five components known to have allergenic properties -
Benzodiazepine Metabolites by Reversed-Phase HPLC (PRP-1)
The separation of metabolites from benzodiazepine use is examined using reversed-phase chromatography. -
Adenine and Guanine Nucleotide Purification (PRP-1)
Using ion-pair reversed-phase HPLC to separate nucleotides ATP, ADP and AMP from like-phosphorylated guanosine metabolic products from tissue extracts. -
Purification of Small Drug Compounds (PRP-1)
Hamilton PRP-1 polystyrene-divinylbenzene RP-column has been compared to two C18 silica stationary phases for the separation of sibutramine, an antiobesity drug, using paroxetine as an internal standard.
Resources
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Reference Guide - HPLC Columns
Learn how to choose a column, how to maximize the life of a column through proper care, cleaning, and restoration.
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How do I restore my HPLC column?
Performance will inevitably degrade as the column is used, however this procedure may restore the performance of a column that has become fouled. Learn more in this step-by-step guide. -
How Do I Connect a Stainless Steel Guard Cartridge to My HPLC Column?
The process of connecting a guard cartridge to an HPLC column is fairly simple, but requires different steps based on the HPLC instrument and the guard cartridge configuration. Click to read our step-by-step guide. -
How Do I Install My HPLC Column?
Connecting an chromatography column is not difficult, but if the proper steps aren't followed, chromatographic results can be compromised. Follow this step-by-step guide to get it done right.
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USP "L" Column Listings
Explore Hamilton’s qualifying USP “L” columns. -
HPLC Guard Columns, HPLC Guard Cartridges
HPLC guard columns and guard cartridges protect analytical, semi-prep and preparative HPLC columns. They help by removing particulate contaminants and highly absorptive compounds from samples, prolonging column life.

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