RCX-30: High Exchange Capacity Anion Exchange HPLC Columns
Hamilton RCX-30 columns are designed with high exchange capacity for quality carbohydrate separation. Isocratic or gradient separations can be used depending on the complexity of the mixture. A pulsed amperometric detector (PAD) is recommended for gradient separations to utilize the full potential of this column. High pH can be used to enhance carbohydrate separation on RCX-30 columns due to the negative charge of sugars at basic pH.
Application Examples: Complex mono- and disaccharide separation.
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RCX-30 Columns
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RCX-30 Guard Columns
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RCX-30 Bulk Resin
RCX-30 Overview
Hamilton RCX-30 columns have further increased exchange capacity from RCX-10 columns. This results in longer retention and better separation of carbohydrate mixtures. High pH can be used to enhance carbohydrate separation on RCX-10 and RCX-30 columns due to the negative charge of sugars at basic pH.
How to Choose Column Specifications for Your Application
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Specific Applications
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Glucose in Parentarel Nutrition by Anion Exchange HPLC (RCX-30)
Establishing an accurate standard method of analysis for the determination of total glucose content in parenteral nutrition solutions. -
Saccharide Identification in Packaged Foods by Anion Exchange HPLC (RCX-30)
With the rise of diabetes and other metabolic diseases, nutritional content has found itself at the forefront of consumer concern.
Resources
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Reference Guide - HPLC Columns
Learn how to choose a column, how to maximize the life of a column through proper care, cleaning, and restoration.
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How do I restore my HPLC column?
Performance will inevitably degrade as the column is used, however this procedure may restore the performance of a column that has become fouled. Learn more in this step-by-step guide. -
How Do I Connect a Stainless Steel Guard Cartridge to My HPLC Column?
The process of connecting a guard cartridge to an HPLC column is fairly simple, but requires different steps based on the HPLC instrument and the guard cartridge configuration. Click to read our step-by-step guide. -
How Do I Install My HPLC Column?
Connecting an chromatography column is not difficult, but if the proper steps aren't followed, chromatographic results can be compromised. Follow this step-by-step guide to get it done right.
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USP "L" Column Listings
Explore Hamilton’s qualifying USP “L” columns. -
Choosing Column Specifications
The sample amount needed to be purified or separated for analysis will dictate which scale of chromatography is needed. In general, the scales of chromatography are microbore, analytical, semi-preparative, and preparative. Nanoscale and microscale are smaller scale ranges but are not covered in Hamilton’s product offerings. READ MORE
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