PRP-X500: Methacrylate (SAX) Anion Exchange HPLC Column

Hamilton PRP-X500 HPLC Column

PRP-X500 is a superficially porous polymeric anion exchange column designed for the separation, purification and isolation of proteins, peptides and DNA/RNA. The methacrylate polymeric coating of the PRP-X500 provides a more hydrophilic surface, preventing hydrophobic interaction sample losses.
 

Application Examples: Proteins, peptides, and DNA/RNA.

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PRP-X500 Columns

Both fast separations and good sample capacity are achievable with PRP-X500 columns.
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PRP-X500 Guard Columns

Protect your PRP-X500 column.
Guard columns

PRP-X500 Bulk Resin

Pack your own column.
Bulk resin

PRP-X500 Overview

Fast separations and good sample recovery

PRP-X500 is a superficially porous polymeric anion exchange column designed for the separation, purification and isolation of proteins, peptides and DNA/RNA. The methacrylate polymeric coating of the PRP-X500 provides a more hydrophilic surface, preventing hydrophobic interaction sample losses typically seen on other commercially available protein HPLC columns.

The non-porous nature of the packing material improves mass-transfer, shortening run times and improving resolution. Both fast separations and good sample capacity are achievable with PRP-X500 columns.

Sample recovery is excellent with PRP-X500 and the support’s limited permeability prevents proteins from entering the pores and unfolding, which causes peak ghosting. The superficially porous properties of the packing material improves mass-transfer, shortening the diffusion path of the analyte. This results in shortened run times, improved resolution, and sharp sample bands.

Application Examples: Proteins, peptides, and DNA/RNA.

Tech Center

HPLC Column Care & Troubleshooting
HPLC Resin Specifications
HPLC Guard Cartridge Holder Assembly - PEEK
Safety Data Sheets
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PRP-X500: Methacrylate (SAX) Anion Exchange HPLC Column Resources

Browse app notes, user guides, specification documents, and more in our Knowledge Center.

Browse app notes, user guides, specification documents, and more in our Knowledge Center.

HAM SDS PRP X500 resin dry

SDS

SDS - PRP-X500 (resin, dry)

Download the Safety Data Sheet for Hamilton PRP-X500 Resin (Dry), used as a filter media in HPLC applications. Includes key safety, handling, and first aid information.
HAM Reference Guide HPLC Columns

Guide

Reference Guide - HPLC Columns

Learn how to choose a column, how to maximize the life of a column through proper care, cleaning, and restoration.

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Explore Hamilton’s qualifying USP “L” columns.

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HPLC FAQ

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How Do I Install My HPLC Column?

Connecting a chromatography column is not difficult, but if the proper steps aren't followed, chromatographic results can be compromised. Follow this step-by-step guide to get it done right.
HPLC FAQ

FAQ

How Do I Connect a Stainless Steel Guard Cartridge to my HPLC Column?

The process of connecting a guard cartridge to an HPLC column is fairly simple, but requires different steps based on the HPLC instrument and the guard cartridge configuration. Click to read our step-by-step guide.
HPLC FAQ

FAQ

How do I restore my HPLC column?

Performance will inevitably degrade as the column is used, however this procedure may restore the performance of a column that has become fouled. Learn more in this step-by-step guide.
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