BIOSPOT Washing Protocols for Ultra-Low-Volume Assays

Hamilton application note cover titled "Optimizing Washing Protocols on the BIOSPOT® Ultra-Low-Volume Dispensing System"

Validating Washing Protocols for Ultra-Low-Volume Dispensing

Reusing consumables in ultra-low-volume workflows requires validated washing strategies that balance contamination control with assay performance. This application note demonstrates how the BIOSPOT® ultra-low-volume dispensing system enables configurable and validated washing protocols using a structured framework based on fluorescence and qPCR assays. The approach allows users to define application-specific cleaning procedures without compromising sensitivity or reproducibility.

  • Effective carryover reduction: Residual signal was reduced to less than 0.025% after three wash cycles under tested conditions.
  • Validated contamination control: qPCR assays confirmed no detectable DNA carryover across optimized washing protocols.
  • Flexible workflow optimization: Users can define washing strategies based on assay sensitivity, liquid properties, and acceptance criteria.

Read the Full Application Note to Learn More.