The Microlab 600 Series Diluter
The Microlab 600 is a highly precise syringe pump with a graphical user interface designed to quickly and easily dilute and dispense liquids. This positive displacement system provides better than 99% accuracy, independent of a liquid's viscosity, vapor pressure and temperature. The inert fluid path minimizes sample carry over and improves compatibility with harsh chemicals.
If you are planning to perform the same dilution all the time then select syringes with nominal volumes closest to the desired sample and diluent volume. If you are planning to do a variety of different applications with the same instrument setup then compromises in dispense volume and accuracy should be considered carefully. Contact a Hamilton Specialist to make the best selection.
An accuracy of 1% and 0.2% precision is achieved when dispensing above 10% of a syringes nominal volume. Reasonable accuracy can be achieved down to 1% of a syringes nominal volume. The Microlab 600 syringe allows for programming of dispense volumes in increments less that 0.01% of the nominal syringe volume.
Sample Syringe Considerations
When selecting a sample syringe it is important to consider the hand probe. When using a Disposable Tip Hand Probe (DTHP) the maximum tip volume is 1 mL so it would not make sense to select a larger sample syringe. With the Concorde Hand Probe the syringe should not exceed 80% of the volume of the dispense tubing. Longer custom tubing assemblies are available if a large sample size is required.
Diluent Syringe Considerations
When selecting a diluent syringe it is important to keep in mind the desired final volume of your dilution. It is also critical to consider priming of the instrument. While it is possible to select a 10 µL syringe it could take a very long time to prime the fill and dispense tubing with such a small syringe. In practice a 1 mL or larger syringe is preferred.
This is method is available with both the 'Basic' and 'Advanced' controller. It is the fastest way to program a dilution method but has the least options and functionality. Set a volume for the left diluent syringe and the right sample syringe and you are ready to go!
The dilution wizard is available in the 'Advanced' controller. It provides a flexible interface for entering dilutions by sample volume, ratio, or final volume. Wizard settings can be saved as favorites or can be stored as a custom method. Additional wizard features include:
- Air Gap between sample and diluent or after sample is aspirated
- Wash volume to rinse after a dilution
- Syringe Aspirate and Dispense Speeds
Dilution with a Disposable Tip
For biological samples where cells are cultured or DNA is amplified a disposable tip may be preferred. In this case the sample is aspirated and dispensed using the disposable tip which is then discarded prior to dispensing the diluent.
Standard Preparation from Powder
Preparation of standards for analytical analysis usually requires precise weighing and transferring of powders into volumetric glassware. The Dry Weight Dilution Wizard enables weighing directly into a vial and automatic calculation of the liquid volume needed to make the desired concentration. No more transfer errors and cumbersome calculations.
Guide - Microlab 600 Quick StartMicrolab 600 Quick Start Guide
Technical Note: Microlab 600 Predicted Dispense Volume vs Gravimetric MeasurementMicrolab 600 Predicted Dispense Volume vs Gravimetric Measurement
Instructions - Gastight Instrument SyringesHamilton Gastight Instrument Syringes are precision, measuring instruments.
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